Targeted Sequencing

Targeted sequencing is a rapid and cost-effective way to detect known and novel variants in selected sets of genes or genomic regions. Gene sequencing can be accomplished using several different DNA sequencing methods, depending on the scale. Highly targeted single-gene sequencing is accurate, fast, and affordable with Sanger sequencing. Fast and affordable sequencing of multiple genes and panels can be accomplished with the Ion PGM™ System combined with Ion AmpliSeq™ technology.

Pre-Designed Primers for PCR and Sanger Sequencing

Targeted sequencing requires upfront selection and isolation of genes or regions of interest, typically by either PCR amplification or hybridization-based capture methods. For sequencing a small number of targeted regions, PCR amplification is used in conjunction with Sanger sequencing. For larger numbers of genes or regions, the Ion AmpliSeq™ gene panels offer the ability to easily target and sequencing hundreds of genes in one day on the Ion PGM™ System. For higher density target regions of up to ~60 MB, the Ion TargetSeq™ Enrichment System is a cost-effective, fully customizable in-solution capture method for the Ion PGM™ System..

• Ion AmpliSeq™ Target Selection Technology
• Ion TargetSeq™ Custom Enrichment Kit

Sanger sequencing is the gold standard for sequencing technology that is very flexible for sequencing highly focused sets of genes or regions. With long read capabilities, up to 1000 bp, Sanger sequencing is ideal for highly accurate targeted sequencing of larger regions. Designing experiments is now easier than ever with our new Primer Designer™ tool for Sanger sequencing (currently available in the US & Canada), containing >300,000 pre-designed primer pairs for PCR and sequencing.

The Ion PGM™ sequencer provides the fastest sequencing runs for targeted sequencing across multiple genes and multiple samples, when compared to other sequencing technologies. Various options for library preparation and the ability to scale with chips of varying sequencing capacity allow you to meet diverse project requirements.