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Immunology Research Using Targeted NGS
Deeper understanding of the adaptive immune system powers better insights in its role in health and disease, leading to crucial developments in immunotherapies. The Ion Torrent immunology research portfolio enables a multi-dimensional research approach to repertoire sequencing and immune response. Each targeted next-generation sequencing (NGS) assay is powered by the accuracy and sensitivity of Ion Torrent technology to help discover more from within precious samples.
Learn about NGS-based immune cell repertoire analysis for human disease research. Watch webinar ››
- TCR beta assays for deeper insights in clonality and convergence
- BCR IgH assays to quantify somatic hypermutations and study isotypes
- Immune response assay to detect low expression genes
For mouse studies:
- Sequencing assays to interrogate CDR3 for TCR beta and for BCR IgH, enabling deeper insights in repertoire analysis.
Additionally, factors like gene expression and microbiota influence the immune system. Transcriptome analysis and analysis of the human microbiome are also enabled through Ion Torrent NGS.
Click image to enlargeIon Torrent targeted NGS for immunology research. Comprehensive solution with simple, fast, scalable and streamlined workflow
Video: Immunological biomarker research to predict response to therapy and disease progression
Watch Dr. Carl Goodyear from the University of Glasgow discuss the importance of molecular profiling and how immune repertoire sequencing with Ion Torrent address this need for rheumatoid arthritis and SARS-CoV-2 research.
Ion Torrent next-generation sequencing systems
Scalable targeted NGS to support small and large projects
The Ion GeneStudio S5 system is a scalable, targeted-NGS workhorse with wide application breadth and throughput capability.
Specimen to report in a single day with a hands-off, automated workflow*
The Genexus System is the first turnkey NGS solution that automates the specimen-to-report workflow and delivers results in a single day with just two user touchpoints.*
Immunology NGS assays for human research samples
Oncomine TCR Beta-LR Assay
Uses long read sequencing technology to efficiently capture all three complementarity determining regions of the TCR beta chain (CDR1, CDR2, CDR3) with high accuracy. RNA sample input allows sequencing of productive and relevant VDJ rearrangements, improving the identification of rare clones in research samples.
Key applications:
Biomarker discovery
TCR convergence
Assess clonality
Oncomine TCR Beta-SR Assay
Compatible with both FFPE DNA and RNA, this short-read sequencing assay specifically interrogates the CDR3 region of the TCR beta chain and enables characterization of the immune status and detection of potential biomarkers in T cell measurable residual disease (MRD) in peripheral blood.
Key applications:
Assess clonality and convergence
MRD
Oncomine BCR IGH LR Assay
Uses the long read sequencing capabilities of Ion Torrent technology to interrogate all complementarity determining regions (CDR1, CDR2, and CDR3) of the BCR immunoglobulin heavy chain (IgH) in research samples.
Key applications:
Assess clonal expansion and evaluate clonal evolution
Quantify somatic hypermutations
Evaluate isotype abundance
Oncomine BCR IGH SR Assay
Study the CDR3 region of the BCR immunoglobulin heavy chain (IgH) from research samples such as FFPE or circulating free nucleic acid in blood. The assay provides accurate data on isotype representation, which may be of importance in biomarker research to indicate disease or immunodeficiency.
Key applications:
Identify somatic hypermutation in the diverse CDR3 region
Study clonal evolution and isotype abundance
MRD
Oncomine Immune Response Research Assay
The assay detects lower expressors associated with T cell receptor (TCR) signaling and check-point inhibitors in research samples, thereby allowing researchers to focus on the correct TCR and the most suitable immunotherapy.
Key applications:
Biomarker profiling
Sample stratification
Immunology NGS assays for mouse samples
Ion AmpliSeq Mouse TCR Beta SR Assay
Designed to accurately identify and measure the clonal expansion of T cell lymphocytes from mouse models. The panel identifies T cell clones by targeting the complementarity-determining region 3 (CDR3) using either DNA or RNA from a variety of samples such as blood, PBMCs, and FFPE samples.
Key applications:
Assess clonal expansion and convergence
Identify T cells that are responding to antigenic challenge
Ion AmpliSeq Mouse BCR IGH SR Assay
A highly accurate sequencing solution for examining the CDR3 region and expanding your B cell studies in disease and immunotherapy research.
Key applications:
Identify somatic hypermutations
Longitudinal analysis and research biomarkers to evaluate vaccine efficacy