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NGS Masters: Consistent NGS Library Prep Success at a Lower Cost
Invitrogen Collibri next-generation sequencing (NGS) libraries prepared on the SPT Labtech mosquito HV automation platform provide reliable statistical power to interpret results and reduce NGS library prep costs by 6-fold.
- Miniaturized reagent volumes reduce the cost of NGS library prep by 6-fold compared to manual protocols and traditional automated protocols
- Consistent, reliable variant detection from as little as 1 ng of DNA
- Improved sequencing success through the preparation of sample replicates at no additional preparation cost
Reduce the cost of NGS library prep compared to traditional automation
The mosquito and dragonfly automation systems from SPT Labtech efficiently generate NGS libraries at a fraction of the traditional volumes, up to a 10-fold reduction. Miniaturization provides cost savings while maintaining the quality of the Collibri libraries for Illumina NGS systems.
Save time by using automated scripts for the Collibri portfolio of library prep kits and accessories, developed by SPT Labtech.
For users requiring higher throughput the dragonfly discovery can be used alongside the mosquito HV for all reagent dispensing steps. The dragonfly discovery enables rapid non-contact dispensing of reagents whilst also significantly reducing plastic consumable consumption.
| Collibri ES DNA Library Prep Kit for Illumina Systems |
Supported, documentation available
| Dispensing steps supported |
|---|---|---|
| Collibri Library Quantification Kit |
Push-button setup using the wizard
| Dispensing steps supported |
Reliable, consistent variant detection from 1 ng of DNA
When automating NGS library prep methods, it is critical to maintain even coverage and minimize bias. GC bias is the biggest contributor to uneven coverage, but it can be minimized by using an optimal PCR master mix or PCR-free library preparation methods. Collibri DNA library preparation kits contain Collibri Library Amplification Master Mix to enable uniform coverage across a wide range of GC content. Representative fastq files are available via Connect, our cloud-based platform, for analysis with your analytical pipeline.
Figure 1. Call variants with confidence. Coriell NA12878 DNA was converted into a library using the Collibri PS DNA Library Prep Kit for Illumina Systems and sequenced on an Illumina™ NovaSeq™ 6000 system at 372M reads per sample. SNP recall calculated using FreeBayes v1.2.0.
Improved sequencing success rates
Miniaturization decreases the volume of NGS library prep reagents by as much as 10-fold, enabling 5–6 replicates of each sample to be generated for less than the cost of a single sample using traditional automation. Sequencing 5-6 replicates per sample removes the need to repeat the library preparation process or sequencing run if a single sample is not successfully sequenced. The likelihood of a sample being successfully sequenced the first time is therefore improved.
Increasing NGS experimental power without increasing costs
Dr. Stuart Levine, Director of the BioMicro center at MIT, has developed methodologies to dramatically lower NGS library prep costs without sacrificing data quality. Performance data from the Collibri ES DNA Library Prep Kits
demonstrates 10–15 fold reduction in costs.
Visual feedback at each step during NGS library prep confirms well-to-well consistency
Each reagent in the Collibri portfolio of NGS library prep kits and accessories contains a tracking dye to visualize library prep progress. When reagents are thoroughly mixed, the solutions change color to indicate proper library preparation technique. Each step in the library prep process contains an internal control in the form of reagent colors, improving the success rate of NGS library generation. The tracking dyes are removed prior to library quantification and do not interfere with sequencing.
