Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
La reprogramación de células somáticas a células madre pluripotentes inducidas es un paso esencial y que potencialmente requiere mucho tiempo en la investigación con células madre. Es por eso que ofrecemos opciones de tecnologías y servicios de reprogramación sin integración que se ajustan a sus necesidades y recursos de investigación: puede hacerlo usted mismo o dejar que lo hagamos por usted.
Ahora disponible:medio Essential 6™ para reprogramación sin dosificador
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| Episomal iPSC Reprogramming Vectors* | Epi5™ Episomal iPSC Reprogramming Kit** | CytoTune®-iPS Sendai Reprogramming Kit | CytoTune®-iPS 2.0 Sendai Reprogramming Kit | |
|---|---|---|---|---|
| Description | Proven technology for viral-free iPSC generation | High efficiency viral-free system requiring no small molecules | Efficient generation of integration-free iPSCs from multiple somatic cell types | Highest efficiency integration-free reprogramming system |
| Reprogramming Efficiency | 0.002–0.08% | 0.04–0.3% | 0.01-0.5% | 0.02 – 1.2% |
| Genes Utilized | Thomson/Yamanaka factors | Yamanaka factors +Lin28 | Yamanaka factors | Yamanaka factors |
| Method & Medium for Reprogramming Fibroblasts | Electroporation using Neon® in N2/B-27 Medium + small molecules | Electroporation using Neon® in N2/B-27 Medium | Add CytoTune® to cells in fibroblast medium | Add CytoTune® 2.0 to cells in fibroblast medium |
| iPSC Growth Medium | Day 15: Transfer to Essential 8™ Medium | Day 15: Transfer to Essential 8™ Medium | Day 7: Transfer to Essential 8™ Medium or KnockOut™ Serum Replacement supplemented medium | Day 7: Transfer to Essential 8™ Medium or KnockOut™ Serum Replacement supplemented medium |
| Genomic Integration-free | ✓ | ✓ | ✓ | ✓ |
| Virus-free Reprogramming | ✓ | ✓ | ||
| Blood Cell Reprogramming | ✓ | ✓ | ✓ | ✓ |
| Transfection Products | Neon® Transfection System or similar device | Neon® Transfection System or Lipofectamine® 3000 | None | None |
| Available in CellModel™ Services | ✓ | ✓ | ✓ | |
| Viral Clearance | N/A | N/A | Up to 15 passages | Up to 5 passages |
| Reporter Available | N/A | N/A | CytoTune®-EmGFP Sendai Fluorescence Reporter | CytoTune®-EmGFP Sendai Fluorescence Reporter |
*Commercialized in Partnership with Cellular Dynamics International.
**Designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University