Nucleic Acid Detection and Analysis—Chapter 8

Nucleic Acid Stains—Section 8.1

• Properties of Cyanine Dyes
• Premier Cyanine Dyes for Ultrasensitive Nucleic Acid Detection and Quantitation
• Cell-Impermeant Cyanine Dimers: The TOTO Family of Dyes
• Cell-Impermeant Cyanine Monomers: The TO-PRO Family of Dyes
• Cell-Impermeant SYTOX Dyes for Dead-Cell Staining
• Cell-Permeant Cyanine Dyes: The SYTO Nucleic Acid Stains
• Amine-Reactive Cyanine Dye
• Phenanthridines and Acridines: Classic Intercalating Dyes
• Indoles and Imidazoles: Classic Minor Groove–Binding Dyes
• Other Nucleic Acid Stains

Labeling Oligonucleotides and Nucleic Acids—Section 8.2

• ChromaTide Nucleotides
• Amine-Modified Nucleotides
• ARES DNA Labeling Kits
• FISH Tag DNA and FISH Tag RNA Kits
• ULYSIS Nucleic Acid Labeling Kits
• Labeled Oligonucleotides
• Other Labeling Methods for Nucleic Acids

Nucleic Acid Quantitation in Solution—Section 8.3

• Quant-iT and Qubit Assay Kits for DNA and RNA
• Qubit 2.0 Fluorometer
  
• PicoGreen dsDNA Quantitation Assay
• OliGreen ssDNA Quantitation Assay
• RiboGreen RNA Quantitation Assay
• Other Stains for Nucleic Acid Quantitation in Solution
• Real-Time Quantitative PCR Using SYBR Green I Nucleic Acid Gel Stain

Nucleic Acid Quantitation on Gels, Blots and Arrays—Section 8.4

• Nucleic Acid Detection in Gels
• Safe Imager Blue-Light Transilluminator
• SYBR Photographic Filters
• Capillary Electrophoresis and Channel Electrophoresis
• Quality Control Testing on Microarrays and Blots

Related Tables

• Specialty nucleic acid reagents for molecular biology—Table 8.1
• Cell membrane–impermeant cyanine nucleic acid stains—Table 8.2
• Cell-permeant cyanine nucleic acid stains—Table 8.3
• Properties of classic nucleic acid stains—Table 8.4
• Characteristics of Molecular Probes ChromaTide and aha labeled nucleotides—Table 8.5
• Molecular Probes nucleic acid labeling kits—Table 8.6
• Amine-reactive dyes for nucleic acid sequencing—Table 8.7
• Selection guide for the Qubit and Quant-iT Assay Kits—Table 8.8

Related Technical Notes

• SYBR Safe DNA Gel Stain—Note 8.1