Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
This kit enables analysis of sperm viability and fertilizing potential. SYBR 14 labels live sperm with green fluorescence, and propidium iodide labels membrane-compromised or dead sperm with red fluorescence.
1. Dilute semen sample in Live Cell Imaging Solution plus 10% BSA
2. Add 900 µL DMSO to SYBR 14 dye to make a stock solution
3. Add 1 µL of SYBR 14 stock solution and 5 µL of propidium iodide solution to 1 mL of diluted semen
4. Incubate for 5–10 minutes at 37°C
5. Analyze on a flow cytometer
SYBR 14 | Propidium iodide | |
---|---|---|
Excitation/Emission | 488/516 nm | 535/617 nm |
Flow cytometer channel | FITC | RPE |
Storage conditions | –20°C | –20°C |
Bovine sperm labeled with the LIVE/DEAD Sperm Viability Kit.