Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
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The forced proximity test helps to assess the quality of the biotinylated antibody. If it does not pass, then either the antibody is under-biotinylated, or there still is free biotin in the solution. Even as little as 80 nM free biotin can cause the test to fail. Make sure to dialyze the antibody well (change the buffer at least five times, including one overnight exchange). We recommend using the Slide-A-Lyzer® Mini Dialysis Unit. Repeat the dialysis steps and perform the test again.
If your protein target is not on the list, you can create your own antibody probes with the antibody of your choice using the TaqMan® Protein Assays Open Kit. If your antibody is not biotinylated, you will have to biotinylate it before use with the Open Kit.
There may be several reasons for not seeing any change in expression. Check for the following:
There may be several reasons for not seeing any change in expression. Check for the following:
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