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Optimize your experiments with the QuantStudio Absolute Q Digital PCR Systemto get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs.
View the relevant questions below:
For QuantStudio Absolute Q instrument specifications, please see Appendix F of the QuantStudio Absolute Q Digital PCR System Installation, Use, and Maintenance Guide.
MAP stands for patented Microfluidic Array Plate technology. The QuantStudio Absolute Q Digital PCR instrument uses QuantStudio Absolute Q MAP16 Digital PCR Plates (MAP plates) for loading samples and running experiments. The MAP plates use microinjection-molded plate technology to overcome common reagent distribution challenges of inconsistency and high dead volume. This novel approach provides many benefits, including precise micro-chamber volume, consistent sample digitization, and greater than 95% of the loaded volume analyzed:
Each microchamber holds 432 picoliters (pL).
The QuantStudio Absolute Q MAP16 Digital PCR Plate is a microinjection-molded plate which is 85.5 mm x 128 mm and consists of 16 microarrays. Each microarray consists of approximately 20,000 microchambers, each accommodating 432 pL (picoliter) volume of reaction. See image that is taken from page 29 of the QuantStudio Absolute Q Digital PCR System Installation, Use, and Maintenance Guide.
No. Microarrays will be used in columns of 4 and unused columns can be used at a future time.
Yes, ROX dye is the passive reference dye for QC (Quality Control).
The QuantStudio Absolute Q Digital PCR System has filters that support FAM, HEX (not field calibrated), VIC, ABY, ROX (QC channel), CY5, and JUN dyes.
The .zst data files are not automatically generated. To create a .zst file, select the run by checking the box to the left of the run name and then click on the “Export Run” button that appears. You will be asked if that is the run you would like to export and then would need to select a location to export the file to.
The QuantStudio Absolute Q Digital PCR System is meant to be used with the Absolute Q DNA Digital PCR Master Mix (5X) (Cat. No. A52490) or Absolute Q 1-step RT-dPCR Master Mix (4X) (Cat. No. A55146). Other master mixes have not been validated with this system.
Yes. Here are some precautions to take:
For more detailed instructions, please see the guidelines in Chapter 2 of the QuantStudio Absolute Q Digital PCR System Installation, Use, and Maintenance Guide.
A result can be provided in less than 90 mins depending on the protocol that is used.
QuantStudio Absolute Q Digital PCR Software can only manage one QuantStudio Absolute Q Digital PCR instrument at a time.
The QuantStudio Absolute Q MAP16 Digital PCR Plate can accommodate up to 16 individual samples. Loading the same sample in multiple microarrays as replicates or for the purpose of pooling results will decrease the number of individual samples that can be run on the same plate.
Please see the sections on determining the optimal dilution in Chapter 2 of the QuantStudio Absolute Q Digital PCR System Installation, Use, and Maintenance Guide.
9 µL of sample is loaded into each well along with 15 µL of isolation buffer. Loading more or less of either the sample or isolation buffer will affect filling of the microchambers and the run results.
For Research Use Only. Not for use in diagnostic procedures.