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Subunit Mass Analysis
Subunit middle-down analysis
Monoclonal antibody (mAb) subunit analysis involves the antibody fragmentation through digestion and reduction of IgG antibody molecules. The cysteine protease produced by S.pyogenes, known as IdeS, is highly specific. IdeS digestion followed by reduction generates three subunits that are readily separated by reversed-phase (RP) UHPLC using Thermo Scientific MAbPac RP columns. Deconvolution of subunit mass spectrometry (MS) spectra is easy with powerful Thermo Scientific BioPharma Finder software.
Popular subunit mass analysis products
Subunit mass analysis workflow
Antibody fragment types
Fab Preparation Kit
Thermo Scientific Pierce Fab Preparation Kit uses immobilized papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc antibody fragments and subsequently to purify the Fab using Protein A agarose.
Subunit mass analysis categories
High-resolution separations
Use Thermo Scientific MAbPac RP columns for high-performance, reversed-phase HPLC and LC/MS characterization of mAbs, antibody fragments, charge variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins.
Chromatography built for biopharma
The innovative architecture of the new Thermo Scientific Vanquish Flex UHPLC system is tailored to the specific and demanding analytical requirements that these essential drugs impose. Vanquish Flex UHPLC has been designed and manufactured to exacting engineering standards to be truly built for biopharma.
Protein reduction
Choose between research grade dithiothreitol (DTT) or Tris-(2-Carboxyethyl)phosphine, (TCEP) for antibody reduction.
State-of-the-art intact protein and subunit analysis
The Thermo Scientific Orbitrap Exploris 240 mass spectrometer provides a complete BioPharma characterization solution. The extreme resolving power of the Thermo Scientific Orbitrap mass analyzer ensures isotopic resolution of monoclonal antibody subunits and facilitates middle-down sequencing.
State-of-the-art intact protein and subunit analysis
The sliding window method and Thermo Scientific Xtract deconvolution algorithm within Thermo Scientific™ BioPharma Finder™ software enables monoisotopic mass determination of monoclonal antibody subunits.
Featured video
Orbitrap for native mass spectrometry providing Omass with a new perspective in drug discovery.
Featured intact protein analysis resource
Intact and Native Characterization webinars
Join experts from Genentech and Thermo Fisher to get great structural insight into your drug product using Orbitrap high resolution accurate mass spectrometers.
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