Digital PCR Workflow Solutions for Analytical Testing

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Complete digital PCR workflow for quality control applications

Realize the true power of digital PCR with Applied Biosystems SEQ Analytical Assay Kits and the Applied Biosystems QuantStudio Absolute Q Digital PCR System. This powerful combination offers an advanced solution that helps deliver the high performance you need with the workflow simplicity you want, making it a valuable tool for precision and accuracy in quality control applications.

  • Confidence in results: Accurate, sensitive quantitation of your target of interest using Applied Biosystems TaqMan chemistry
  • Fast time to answers: Streamlined digital PCR workflow helps deliver results in ~3 hours
  • Regulatory compliant: Assays designed to meet regulatory guidelines from the FDA, WHO, EMA, and other regulatory agencies
  • Global support network: Supported throughout the validation process by our regulatory and field application specialists

Streamlined workflow—From set up to analysis in less than 3 hours

Our end-to-end SEQ digital PCR solution features comprehensive assay kits complete with TaqMan primer/probe sets and all necessary reagents, designed to run on the QuantStudio Absolute Q Digital PCR System with integrated analysis software for simplified data output. For exceptional workflow and throughput flexibility, the Absolute Q offers optional “walk-away” automation solutions. And for the few applications that require sample preparation, we offer complementary Applied Biosystems PrepSEQ kits designed to deliver reliable, consistent recovery of target DNA and RNA from matrices varying in complexity, with both manual and automated workflow options.

Expanding menu of digital PCR assay kits for analytical testing

Residual Host Cell DNA Quantitation

The portfolio of Applied Biosystems resDNASEQ Residual DNA Kits for digital PCR continues to grow, providing coverage for many commonly used cell lines in biotherapeutic development.

ViralSEQ Viral Titer Determination

Applied Biosystems ViralSEQ dPCR Viral Titer Assay kits are designed for precise and consistent virus quantitation and characterization with solutions for both lentivirus and AAV.

Discover powerfully simple digital PCR

The QuantStudio Absolute Q Digital PCR System is a single instrument, plate-based dPCR platform that enables all the necessary steps for dPCR—including data analysis. The dPCR workflow is identical to the qPCR workflow you are already familiar with, allowing for ease-of-use, minimizing hands-on steps, and maximizing consistency.

Simplify data analysis with integrated instrument and analysis software

The Applied Biosystems QuantStudio Absolute Q Software offers robust analysis features and plate-run management tools, allowing for the organization of group sets, plate-run batches, and pre-run analysis threshold settings, along with dilution factor adjustments.

  • Interfaces with the Applied Biosystems QuantStudio Absolute Q Digital PCR System
  • Simplifies operation with streamlined run set-up templates and pre-set thresholding
  • Security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance

Learn more about QuantStudio Absolute Q Software >

Understanding digital PCR and how it compares to qPCR

Video: What's the difference between dPCR and qPCR?         

Webinar: An introduction to dPCR and the QuantStudio Absolute Q system

Infographic: Comparing real-time and digital PCR technologies

Get consistent recovery regardless of sample matrix complexity

Get reliable, consistent quantitative recovery of your target DNA and RNA with a comprehensive solution that is built on years of successful performance. Applied Biosystems PrepSEQ technology enables consistent recovery from matrices varying in complexity, whether it is pH, salt quantity, or protein balance. The nucleic acid extraction workflow includes our proprietary magnetic particle and binding solution that not only enables high binding but complete elution of the purified sample.
 

Discover the efficiency of PrepSEQ sample prep solutions >

Increase sample processing throughput

The KingFisher Apex magnetic particle processor instrument offers an easy workflow with high throughput so you can process up to 96 samples with 1 hour of total hands-on time using PrepSEQ sample preparation kits.

  • Consistent, high-quality nucleic acid recovery from a wide variety of sample types
  • Validated nucleic acid purification method for the resDNASEQ workflow to enable consistent, reproducible results
  • Cross-contamination control leveraging the bead transfer technology

Learn more about sample preparation and automation >

Request a technical consultation

No matter your questions, we are here to help. Our experienced team of scientists and technical specialists is ready to assist you in choosing the right solution and products. Complete this form to start the conversation.
 

 

Analytics Knowledge Hub


Explore the Analytics Knowledge Hub from Thermo Fisher Scientific, providing a robust collection of webinars, articles, eBooks, infographics, and other digital resources to help you optimize the use of our products in your bioprocess workflow.
 

Additional resources

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