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Affordable & pure, flow compatible cells | Highest purity for molecular assays | Untouched—most physiological relevant cells | |||
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Isolation method | Positive isolation | Depletion or positive isolation | Negative isolation | ||
Starting samples | Any sample (whole blood, spleen, lymph node) | Any sample (whole blood, spleen, lymph node) | Spleen, lymph node cells* | ||
# Cells processed | 2 x 109 cells | 1-2 x 109 cells | 1 x 109 cells | ||
Purity | High >90% | High >90% | Standard >70% | ||
Yield | Standard >60% | Highest >90% | Standard >60% | ||
Viability | >90% | >90% | >90% | ||
Isolation time | ~60min | ~30 min | ~50 min | ||
Isolation temperature | Cold | Cold | Cold | ||
Applications | Cellular assays (cell culture, cell activation/ proliferation, cytokine studies) Molecular: isolation of proteins & nucleic acids | Depletion of unwanted CD8+ T cells or positive isolation for molecular isolation of proteins & nucleic acids | Cellular applications for physiological studies (functional studies, functional studies, adoptive transfer, flow analysis or further sorting) | ||
Bead-free target cells | Yes (but antibody present) | No | Yes | ||
Compatible with flow cytometry /flow sorting | |||||
Shelf life from production date | 2 years | 3 years | 3 years | ||
Required materials | Magnets | Magnets | Magnets | ||
Order Now | Order Now | Order Now |
* Lyse the red cells prior to use to improve the purity