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Dulbecco's Modified Eagle Medium (DMEM)
Culture your cells with Gibco DMEM
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
Gibco DMEM is formulated for superior quality, consistency, and control for your mammalian cell culture.
DMEM composition
Dulbecco's Modified Eagle Medium (DMEM) is modified to contain four times the amino acid and vitamin concentration of original Eagle's Minimal Essential Medium. The original DMEM formulation contained a low concentration of glucose (1 g/L) and sodium pyruvate. However, DMEM is now available in a variety of formulations, including those with higher glucose levels and with or without sodium pyruvate. DMEM also uses a sodium bicarbonate buffer system, allowing for maintenance of physiological pH in a 5–10% CO2 environment. Importantly, DMEM commonly requires fetal bovine serum (FBS) supplementation.
Thermo Fisher Scientific offers a variety of Gibco DMEM formulations to support a wide range of cell culture applications—view an example of a detailed DMEM formulation here. Learn about additional DMEM formulation options in the Cell Culture Media Formulation Tool.
Choose the right DMEM
There are several factors to consider when selecting the right Gibco DMEM composition for your cell culture experiments, including format, supplementation, and additives. Our interactive media formulation tool provides side-by-side comparisons of DMEM formulations, allowing for easy selection of the DMEM you need. Select the following modifications:
- L-glutamine/GlutaMAX supplementation
- Phenol red indicator
- Liquid vs powder formats
- Serum supplementation
- Sodium pyruvate additive
- Glucose additive
- HEPES additive
Innovative DMEM formulations
Take your cell culture to the next level with enhanced DMEM formulations. Explore the following formulations that can help improve your cell viability, serum supplementation, cell imaging, and cold storage constraints.
Advanced DMEM
Gibco Advanced DMEM is enriched with normal-serum constituents and therefore requires 50–90% less FBS supplementation, helping reduce variability in your cell culture and lower your cost.
FluoroBrite DMEM
Gibco FluoroBrite DMEM is a DMEM-based media formulation with background fluorescence that is 90% lower than that emitted by standard phenol red-free DMEM, enhancing the signal-to-noise ratio of fluorophores to visualize even the weakest fluorescent events.
BenchStable DMEM
Gibco BenchStable DMEM is formulated for storage at room temperature, supporting cell proliferation and densities comparable to conventional DMEM while freeing up cold storage space.
DMEM with GlutaMAX supplement
Gibco DMEM with GlutaMAX supplement provides your cells with a stable source of L-glutamine that does not spontaneously degrade in cell culture, improving the health and viability of your cells by helping reduce the accumulation of toxic ammonia.
Custom DMEM
If you require a custom DMEM formulation for your cell culture experiments, our media customization services can provide you with the media you need, including custom components, packaging, and QC testing.
GMP manufacturing
Gibco cell culture products are manufactured in facilities compliant with current good manufacturing practices (GMP) and adhere to a robust quality management system, helping to ensure the consistency, reliability, and high quality you can rely on.
Sustainable solutions
We are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.
Resources
Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.
Gibco Cell Culture Basics
Learn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.
Media Formulation Tool
Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.
Related products
Support
Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.
Need technical support? Contact our expert team for technical and application support of Laboratory Products.
For Research Use Only. Not for use in diagnostic procedures.