Automated Nucleic Acid Extraction Kits

Wastewater surveillance has recently gained significant attention as a useful tool for monitoring the regional spread of infectious disease, including the novel coronavirus and other airborne pathogens. MagMAX Wastewater Ultra Nucleic Acid Isolation Kits offer a whole host of advantages over traditional wastewater surveillance methods. They are easy to use, and you can isolate nucleic acids from concentrated wastewater in as little as 45 minutes. Our viral enrichment protocol using magnetic beads offers an efficient concentration and nucleic acid isolation.

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Saliva can be collected non-invasively and is a common source of DNA for research. Without compromising yield and purity, the MagMAX Saliva gDNA Isolation Kit uses a single step to minimize the time and effort typically required by other methods which may use multiple laborious sample processing steps. Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep.

Learn more about saliva-based research in our “How-To” Guide for Saliva Collection and Testing. This complex biofluid plays a critical role in human physiology and disease pathophysiology.

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HIV-1 RNA can be isolated now from dried blood spots (DBS), a sample type that requires no specialized equipment for transport and can be shipped at ambient temperatures. DBS sampling is a cost-effective solution, with easy transportation protocols, that can extend the reach of global health and surveillance in remote populations. The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots isolates and purifies nucleic acid from dried blood spots (DBS) or plasma. The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots is compatible with KingFisher automated purification systems and downstream assays such as the HIV-1 Genotyping Kit with Integrase.

Steps in a typical genotyping workflow include specimen processing, RNA extraction, RT-PCR, nested PCR, confirmation, purification, cycle sequencing, sequencing clean-up, sequence detection, and data analysis.

Steps to extract DNA and RNA from plant samples includes the following: Disrupt the tissue and homogenize the plant sample, bind nucleic acid with target magnetic beads, wash and elute sample to purify DNA using a magnetic particle processing instrument or manually using microcentrifuge tubes.

Challenges of extracting DNA and RNA from plants can be difficult and require kits specifically designed to mitigate carbohydrates, phenolics, and other compounds found in plant tissues. Cell walls can be difficult to disrupt, and lysates often contain inhibiting compounds such as tannins, phenolics, and complex polysaccharides which may hinder DNA quality, affecting downstream reactions.

For automated high-throughput extraction or manual purification, the MagMAX Plant DNA Isolation Kit is designed to reduce these challenges and effectively extract DNA from a wide variety of plant species. The kit uses MagMAX magnetic bead technology and eliminates the need for phenol/chloroform extraction or alcohol precipitation. The procedures are optimized for the isolation of DNA from 10–100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, age of tissue sample, and storage conditions.