miRNA
  • Ideal for reliably quantitating transcripts longer than 200 nucleotides
  • Detect only the intended noncoding RNA transcript
  • Assays are based on proven TaqMan® Assay technology and use existing assay design and manufacturing infrastructure
  • Available for nearly 30 different species

How it works

Long noncoding RNAs (ncRNAs) are transcripts longer than 200 nucleotides. This definition distinguishes long ncRNAs from small regulatory RNAs such as miRNAs. Rather than potentially detecting different transcript variants at a particular gene locus like TaqMan Gene Expression Assays, TaqMan Non-coding RNA Assays detect only the intended ncRNA transcript.


Real-time PCR gene expression and long non-coding RNA workflow:

iconiconiconiconiconicon
Step 1:
Isolate RNA		Step 2:
Design and optimize primers		Step 3:
Reverse transcription		Step 4:
Amplify cDNA		Step 5:
Run qPCR		Step 6:
Analyze

Isolate RNA

Isolate RNA

Isolate RNA using methods that preserve RNA integrity and expression profiles.

RNA isolation kits
Learn more ›

Single Cell-to-CT Kit
For single cell analysis
Order now ›

Cells-to-CT kits
Rapid, simple protocol for RT-PCR sample prep without RNA extraction
Order now ›

TRIzol Reagent
Prepare high-quality, intact RNA from any biological material
Order now ›

mirVana miRNA isolation kits
Rapid isolation of small RNAs
Order now ›

Design and optimize primers

Design and optimize primers

Not required for Applied Biosystems TaqMan Assays. These assays have passed our industry leading bioinformatics design criteria.

For SYBR primer design: Primer Express

Reverse transcription

Reverse transcription

Convert your RNA into cDNA, over a wide range of RNA concentrations.

SuperScript IV VILO Master Mix
Two-step master mix for RT-qPCR applications - enabling earlier Ct values and improved data reproducibility, even with inhibitor-containing RNA samples.
Order now ›

Other SuperScript reverse transcriptase formats
Learn more ›

Amplify cDNA

Amplify cDNA

Single Tube TaqMan Non-Coding RNA Assays
Find my assay ›

Single Tube TaqMan Gene Expression Assays
Find my assay ›

Single Tube Custom TaqMan Gene Expression Assays 
Find my custom assay ›

TaqMan Gene Expression Array Cards and Plates
Find my assay ›

TaqMan OpenArray Plates
Find my plate ›

TaqMan Real-Time PCR Master Mixes
Find my master mix ›

Custom Primers and Primer Express Software
Design and order custom primers for SYBR Green detection
Find custom primers ›

Run qPCR

Run qPCR

Instruments for real time PCR

Compare instruments ›

QuantStudio 3 Real-Time PCR System
Simple to use for all levels of experience

QuantStudio 5 Real-Time PCR System
Easy controls for optimized experiments

QuantStudio 6 Flex Real-Time PCR System
High flexibility and intuitive design

QuantStudio 7 Flex Real-Time PCR System
High versatility and intuitive design

QuantStudio 12k Flex Real-Time PCR System
Maximum throughput, flexibility, and scalability

Analyze

Analyze

Process and interpret qPCR data with intuitive software data analysis tools for a range of applications

Applied Biosystems qPCR analysis modules
Learn more ›

ExpressionSuite software
Learn more ›

Step 1 : Isolate RNA

Isolate RNA

Isolate RNA

Isolate RNA using methods that preserve RNA integrity and expression profiles.

RNA isolation kits
Learn more ›

Single Cell-to-CT Kit
For single cell analysis
Order now ›

Cells-to-CT kits
Rapid, simple protocol for RT-PCR sample prep without RNA extraction
Order now ›

TRIzol Reagent
Prepare high-quality, intact RNA from any biological material
Order now ›

mirVana miRNA isolation kits
Rapid isolation of small RNAs
Order now ›

Step 2 : Design and optimize primers

Design and optimize primers

Design and optimize primers

Not required for Applied Biosystems TaqMan Assays. These assays have passed our industry leading bioinformatics design criteria.

For SYBR primer design: Primer Express

Step 3 : Reverse transcription

Reverse transcription

Reverse transcription

Convert your RNA into cDNA, over a wide range of RNA concentrations.

SuperScript IV VILO Master Mix
Two-step master mix for RT-qPCR applications - enabling earlier Ct values and improved data reproducibility, even with inhibitor-containing RNA samples.
Order now ›

Other SuperScript reverse transcriptase formats
Learn more ›

Step 4 : Amplify cDNA

Amplify cDNA

Amplify cDNA

Single Tube TaqMan Non-Coding RNA Assays
Find my assay ›

Single Tube TaqMan Gene Expression Assays
Find my assay ›

Single Tube Custom TaqMan Gene Expression Assays 
Find my custom assay ›

TaqMan Gene Expression Array Cards and Plates
Find my assay ›

TaqMan OpenArray Plates
Find my plate ›

TaqMan Real-Time PCR Master Mixes
Find my master mix ›

Custom Primers and Primer Express Software
Design and order custom primers for SYBR Green detection
Find custom primers ›

Step 5 : Run qPCR

Run qPCR

Run qPCR

Instruments for real time PCR

Compare instruments ›

QuantStudio 3 Real-Time PCR System
Simple to use for all levels of experience

QuantStudio 5 Real-Time PCR System
Easy controls for optimized experiments

QuantStudio 6 Flex Real-Time PCR System
High flexibility and intuitive design

QuantStudio 7 Flex Real-Time PCR System
High versatility and intuitive design

QuantStudio 12k Flex Real-Time PCR System
Maximum throughput, flexibility, and scalability

Step 6 : Analyze

Analyze

Analyze

Process and interpret qPCR data with intuitive software data analysis tools for a range of applications

Applied Biosystems qPCR analysis modules
Learn more ›

ExpressionSuite software
Learn more ›

Resources

For research use only. Not intended for any animal or human therapeutic or diagnostic use.