microscopic view of fluorescence-stained cells in culture

Having relevant cellular models is essential to better understand disease biology and developing novel therapeutics.  

CellModel services allows you to take a patient derived pluripotent stem cell and differentiate it into a wide variety of terminal cell lineages. Using our proprietary Gibco differentiation kits and reagents, we can generate a range of cell types from pluripotent stem cells. You can choose to provide your own PSCs, or have us reprogram your somatic cells into iPCSs for you. The choice is yours!

Advantages of the CellModel Differentiation Services

  • Speed:  Obtain your terminally differentiated cells in 4 to 8 weeks.
  • Full transparency:   Detailed protocols provided to you after project completion to demonstrate how we reached each milestone and document which tools we utilized.
  • High-quality products: We leveraged our high quality reagents and media for your projects, all of which can be purchased and used in your own lab to facilitate your post-service projects.
  • Exceptional support: Dedicated project manager providing exception support and frequent communication.

Send us an inquiry

Available services

Our differentiation services offer the following cell types: dopaminergic neurons & progenitors, CNS neurons & progenitors, cardiomyocytes, or custom.

For custom terminally differentiated cell type, we can evaluate existing papers, reproduce customer-supplied protocols, or work with you to develop custom differentiation methods and tools.

Differentiation workflow and timeline

Stem cell differentiation projects are typically completed within 4-8 weeks of initiation.  Actual timelines will vary depending on the unique aspects of each project.

Generate or receive PSC: Following receipt of the PSCs, the cell line is recovered expanded in the starting media conditions.  Adaptation to the appropriate medium and matrix maybe be required prior to starting differentiation for increased efficiency in the workflow.

Differentiation: After the PSCs have recovered and the sample is shown to be free of contaminates, the differentiation process starts using our collection of proprietary Gibco differentiation medias and reagents.

Expand: Following specification, the cells are expanded in preparation for characterization and cryopreservation.

Characterization & Bank: The differentiated cells are stained to confirm the expression of key markers to demonstrate the successful differentiation of the PSCs. Additional characterization steps are available to confirm normal karyotype, identity and differentiation.

Deliver Cell Line: A report containing the final characterization results is drafted and provided to the customer along with the shipment of the cryopreserved cells.

Step 1 : Generate or receive PSC

Generate or receive PSC: Following receipt of the PSCs, the cell line is recovered expanded in the starting media conditions.  Adaptation to the appropriate medium and matrix maybe be required prior to starting differentiation for increased efficiency in the workflow.

Step 2 : Differentiation

Differentiation: After the PSCs have recovered and the sample is shown to be free of contaminates, the differentiation process starts using our collection of proprietary Gibco differentiation medias and reagents.

Step 3 : Expand

Expand: Following specification, the cells are expanded in preparation for characterization and cryopreservation.

Step 4 : Characterization & Bank

Characterization & Bank: The differentiated cells are stained to confirm the expression of key markers to demonstrate the successful differentiation of the PSCs. Additional characterization steps are available to confirm normal karyotype, identity and differentiation.

Step 5 : Deliver Cell Line

Deliver Cell Line: A report containing the final characterization results is drafted and provided to the customer along with the shipment of the cryopreserved cells.

Optional services

Choose from a number of additional services to enable full customization of your stem cell reprogramming project, or continue your project with downstream applications such as stem cell genome editing, differentiation, assay development and screening.

Start your stem cell differentiation project today

Contact us today to discuss your stem cell differentiation project with a technical specialist.

Resources

Support