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CellLight Reagents and Their Targeting Sequences—Table 11.1
Learn more in The Molecular Probes Handbook
- Probes for Actin—Section 11.1
- Probes for Tubulin and Other Cytoskeletal Proteins—Section 11.2
- A Diverse Selection of Organelle Probes—Section 12.1
- Probes for Mitochondria—Section 12.2
- Probes for Lysosomes, Peroxisomes and Yeast Vacuoles—Section 12.3
- Probes for the Endoplasmic Reticulum and Golgi Apparatus—Section 12.4
- Probes for the Nucleus—Section 12.5
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Table 11.1 CellLight reagents and their targeting sequences.
| Target | Targeting Sequence | Ref | GFP (489/508 nm)* | RFP (555/584 nm)* | Handbook Section |
|---|---|---|---|---|---|
| Actin | Human actin | 1 | C10506 † C10582 | C10502 † C10583 | Section 11.1 |
| Tubulin | Human tubulin | 2 | C10509 † C10613 | C10503 † C10614 | Section 11.2 |
| Talin | Human talin 2341–2541 | 4 | C10611 | C10612 | Section 11.2 |
| Chromatin | Histone 2B (H2B) | 5 | C10594 | C10595 | Section 12.5 |
| Mitochondria | Leader sequence of E1α pyruvate dehydrogenase | 6 | C10508 † C10600 | C10505 † C10601 | Section 12.2 |
| Lysosomes | Lamp1 (lysosomal-associated membrane protein 1) | 7 | C10507 † C10596 | C10504 † C10597 | Section 12.3 |
| Peroxisomes | Peroxisomal C-terminal targeting sequence | 8 | C10604 | Section 12.3 | |
| Early endosomes | Rab5a | 9 | C10586 | C10587 | Section 12.3, Section 16.1 |
| Late endosomes | Rab7a | 9 | C10588 | C10589 | Section 12.3, Section 16.1 |
| Synaptosomes | Synaptophysin | 10 | C10610 | Section 16.1 | |
| Endoplasmic reticulum | ER signal sequence of calreticulin and KDEL (ER retention signal) | 11 | C10590 | C10591 | Section 12.4 |
| Golgi apparatus | Human golgi-resident enzyme N-acetylgalactosaminyltransferase 2 | 12 | C10592 | C10593 | Section 12.4 |
| Nucleus | LSV40 nuclear localization sequence | 13 | C10602 ‡ | C10603 ‡ | Section 12.5 |
| Plasma membrane | Myristoylation/palmitoylation sequence from Lck tyrosine kinase | 14 | C10607 ‡ | C10608 ‡ | Section 14.4 |
| Cytoplasm | No targeting sequence (BacMam GFP transduction control)** | B10383 | Section 14.7 | ||
* Approximate absorption (Abs) and fluorescence (Em) maxima, in nm; GFP (Green Fluorescent Protein) and RFP  (Red Fluorescent Protein) can be imaged using optical filters for fluorescein (FITC) and tetramethylrhodamine (TRITC) dyes, respectively. † These popular CellLight reagents are offered in small unit sizes (100 µL); all others are provided in a unit size of 1 mL. ‡ Also available is CellLight Nucleus-CFP (C10616) and CellLight Plasma Membrane-CFP (C10606), which generate a cyan-autofluorescent fusion protein targeted to the nucleus and plasma membrane, respectively. ** We also offer the CellLight Null (control) reagent (C10615), a negative transduction control for our CellLight targeted fluorescent reagents that comprises a suspension of baculovirus particles lacking mammalian genetic elements. 1. Curr Biol (1997) 7:176; 2. PLoS One (2009) 4:e8171; 3. J Cell Biol (1995) 130:639; 4. Plant J (2003) 33:775; 5. Curr Biol (1998) 8:377; 6. J Biol Chem (2004) 279:13044; 7. J Cell Sci (2005) 118:5243; 8. J Cell Biol (1989) 108:1657; 9. J Biol Chem (2009) 284:29218; 10. J Neurosci (2006) 26:3604; 11. FEBS Lett (1997) 405:18; 12. J Cell Biol (1998) 143:1505; 13. Trends Biochem Sci (1991) 16:478; 14. EMBO J (1997) 16:4983. | |||||
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