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ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable green-fluorescent Alexa Fluor 488 dye. Like other ReadyProbes reagents, the ActinGreen 488 reagent is formulated as a room temperature–stable solution and comes in a convenient dropper bottle.
First, fix and permeabilize cultured cells with a protocol appropriate for your sample. Counterstain as desired. DAPI may be added directly to fixative.
1. Wash the cells 1–3 times in PBS as needed. |
2. Add two drops of ActinGreen 488 ReadyProbes Reagent per milliliter of medium. |
3. Incubate for 30 minutes, protected from light. |
4. Remove the stain solution. |
5. Wash the cells 2–3 times in PBS. |
6. Image the cells. |
ActinGreen 488 | |
---|---|
Excitation/Emission (nm) | 495/518 |
Standard filter set | FITC |
EVOS Light Cube | GFP |
Storage conditions | Room temperature |
Multicolor staining of HeLa cells. HeLa cells were grown on coverslips and were fixed and permeabilized using the Image-iT Fixation/Permeabilization Kit. Cells were then stained to detect actin (green fluorescence from ActinGreen 488 ReadyProbes Reagent, B-catenin (red-orange fluorescence), and nuclei (blue fluorescence).