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NovaFluor Yellow 570 Dye
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| 2 | 561 | 575/26 | 551 | 566 | (in buffer) 5 | flow cytometry |
Invitrogen NovaFluor Yellow 570 dye can be used as an alternative to phycoerythrin (PE) with similar emission spectrum but far less cross-laser excitation by the blue 488 nm laser. NovaFluor Yellow 570 dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels.
The macromolecule-based NovaFluor Yellow 570 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Can serve as a replacement for PE with far less cross-laser excitation
- Produces highly stable fluorescence and is compatible with all buffers tested
Click image to enlarge
Spectral signature of NovaFluor Yellow 570 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 570 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Staining of mouse splenocytes with NovaFluor Yellow 570 conjugated antibody. C57BL/6 mouse splenocytes were unstained (left) or stained with 0.2 µg of CD19 Monoclonal Antibody, NovaFluor Yellow 570 (right). All cells were co-stained with CD3e Monoclonal Antibody, eFluor 450. Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation. Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.