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Invitrogen iBind Western Systems—no shakers, no trays, no timers. Set up in minutes and walk away. The Invitrogen iBind Western Systems are western blot processing benchtop devices that utilize sequential lateral flow (SLF) to perform hands-free blocking, antibody binding, and washes. In less than 3 hours, blots are processed and ready for final detection. You can continue to use your existing chromogenic, chemiluminescent, or fluorescent western blotting protocols, along with your choice of primary antibody or secondary antibody conjugates of HRP, AP, or fluorescent dyes.
iBind Western Systems are small benchtop devices that automate the western blot processing (immunodetection) steps. The iBind Western Systems rely on mechanical pressure generated by the device on a specialized glass fiber matrix iBind Card to generate the sequential lateral flow (SLF) of immunodetection reagents for the blocking, antibody binding, and wash steps involved in the western blot immunodetection workflow.
iBind Western Systems allow all solutions to be prepared and loaded in the device at the start of the procedure, from which all subsequent steps proceed automatically and uninterrupted by sequential lateral flow technology (SLF), i.e., simple capillary action—no electricity or batteries are required.
Two iBind Western Systems are available: the original iBind Western Device, which accommodates the processing of one mini blot, and the iBind Flex Western Device, which accommodates the processing of up to two mini blots, one midi blot, or up to six vertically cut membrane strips at a time. Setup requires only approximately 15 minutes, with no additional hands-on steps until the blot has been processed and ready for the final detection steps.
iBind Western Device | iBind Flex Western Device | |
---|---|---|
![]() | ![]() | |
Mini blot (single) | Yes | Yes |
Mini blot (dual) | No | Yes |
Midi blot | No | Yes |
Vertically cut strips | No | Yes |
The iBind Flex system comes with interchangeable wells, which allows you to run multiple membrane formats and even run different primary and secondary antibody conditions in the same device at the same time.
Using the same antibody conditions
Using the same or different antibody conditions
Using the same or different antibody conditions
Learn how the application of sequential lateral flow technology allows automated western blot processing with the iBind Western System.
Learn how to process midi, mini or strip blots using the iBind Flex and process mini blots using the iBind with this detailed step by step video.
One of the key reagents of a western blot is the primary antibody; however, this reagent also contributes to over 90% of the total cost of the blot. Because the iBind Western Systems can be more sensitive than manual processing methods, you can use up to 80% less primary antibody and achieve similar results.
Using chemiluminescent or fluorescence-based detection methods, you can use up to 80% less primary antibody than manual methods, saving you significant cost per blot.
A 2-fold dilution series of EGF receptor control cell lysate (30 µg, 15 µg, 7.5 µg, 3.75 µg, and 1.875 µg) was used. Proteins were separated using Bolt Bis-Tris Plus gels on the Mini Gel Tank and transferred to PVDF membranes using the iBlot 2 Dry Blotting System. The blots were probed with an anti–phospho-EGF receptor [Tyr1068] (1H12) mouse monoclonal antibody (1:1,000 dilution, equated to 2 µL antibody for the iBind device method and 10 µL antibody for the manual method) followed by a goat anti–mouse IgG (H+L) peroxidase-conjugated antibody (1:360 for iBind device processing (5.55 µL); 1:1,800 for manual method (5.55 µL)). The standard iBind solution kit was used for the chemiluminescence blot (panel C, manual processing and panel D, iBind system processing); the fluorescence detection (panel A, manual processing and panel B, iBind processing) kit was used for fluorescence detection. These results demonstrate that western blots processed on the iBind device show comparable results to those obtained when western blots are processed manually, with lower overall primary antibody requirements for the iBind device.
Comparison of manually processed midi blots vs. midi blots processed with the iBind Flex Western System.
Samples containing GST-tagged recombinant proteins were separated and then transferred to nitrocellulose membranes using the Invitrogen iBlot 2 Dry Blotting System. Blots were probed with identical concentrations of the same pair of primary and secondary antibodies. The primary antibody was rabbit anti-GST diluted 1:500 (8 µL in 4 mL iBind Flex Solution for the iBind system, 40 µL in 20 mL for manual tray incubation). The secondary antibody was goat anti-rabbit HRP diluted 1:600 (6.7 µL in 4 mL iBind Flex Solution for the iBind system, 33.3 µL in 20 mL for manual tray incubation). For final detection, blots were incubated for 5 minutes in Thermo Scientific SuperSignal West Dura Substrate for visualization with an imaging system.
Comparison of manually processed mini blots vs. mini blots processed with the iBind Flex Western System.
Blots were produced by separating samples and then transferred to nitrocellulose membranes using the iBlot 2 Dry Blotting System. Each gel contained 10 lanes loaded with two-fold dilution series of 293 cell extracts (30 µg to 0.06 µg). After immunodetection using the conditions described below, blots were incubated for 5 minutes in Thermo Scientific SuperSignal West Dura Substrate and the signal documented with an imager.
Comparison of 6 manually processed vertically cut blot strips vs. strips processed with the iBind Flex Western System.
Process up to 6 strips with 6 different antibodies combinations at a single time. Blots were produced by separating samples and transferring to nitrocellulose membranes using the iBlot 2 Dry Blotting System.
Our starter kits include everything you need to start processing your western blots using the iBind Western Systems.
iBind Western Systems are small benchtop devices that automate the western blot processing (immunodetection) steps. The iBind Western Systems rely on mechanical pressure generated by the device on a specialized glass fiber matrix iBind Card to generate the sequential lateral flow (SLF) of immunodetection reagents for the blocking, antibody binding, and wash steps involved in the western blot immunodetection workflow.
iBind Western Systems allow all solutions to be prepared and loaded in the device at the start of the procedure, from which all subsequent steps proceed automatically and uninterrupted by sequential lateral flow technology (SLF), i.e., simple capillary action—no electricity or batteries are required.
Two iBind Western Systems are available: the original iBind Western Device, which accommodates the processing of one mini blot, and the iBind Flex Western Device, which accommodates the processing of up to two mini blots, one midi blot, or up to six vertically cut membrane strips at a time. Setup requires only approximately 15 minutes, with no additional hands-on steps until the blot has been processed and ready for the final detection steps.
iBind Western Device | iBind Flex Western Device | |
---|---|---|
![]() | ![]() | |
Mini blot (single) | Yes | Yes |
Mini blot (dual) | No | Yes |
Midi blot | No | Yes |
Vertically cut strips | No | Yes |
The iBind Flex system comes with interchangeable wells, which allows you to run multiple membrane formats and even run different primary and secondary antibody conditions in the same device at the same time.
Using the same antibody conditions
Using the same or different antibody conditions
Using the same or different antibody conditions
Learn how the application of sequential lateral flow technology allows automated western blot processing with the iBind Western System.
Learn how to process midi, mini or strip blots using the iBind Flex and process mini blots using the iBind with this detailed step by step video.
One of the key reagents of a western blot is the primary antibody; however, this reagent also contributes to over 90% of the total cost of the blot. Because the iBind Western Systems can be more sensitive than manual processing methods, you can use up to 80% less primary antibody and achieve similar results.
Using chemiluminescent or fluorescence-based detection methods, you can use up to 80% less primary antibody than manual methods, saving you significant cost per blot.
A 2-fold dilution series of EGF receptor control cell lysate (30 µg, 15 µg, 7.5 µg, 3.75 µg, and 1.875 µg) was used. Proteins were separated using Bolt Bis-Tris Plus gels on the Mini Gel Tank and transferred to PVDF membranes using the iBlot 2 Dry Blotting System. The blots were probed with an anti–phospho-EGF receptor [Tyr1068] (1H12) mouse monoclonal antibody (1:1,000 dilution, equated to 2 µL antibody for the iBind device method and 10 µL antibody for the manual method) followed by a goat anti–mouse IgG (H+L) peroxidase-conjugated antibody (1:360 for iBind device processing (5.55 µL); 1:1,800 for manual method (5.55 µL)). The standard iBind solution kit was used for the chemiluminescence blot (panel C, manual processing and panel D, iBind system processing); the fluorescence detection (panel A, manual processing and panel B, iBind processing) kit was used for fluorescence detection. These results demonstrate that western blots processed on the iBind device show comparable results to those obtained when western blots are processed manually, with lower overall primary antibody requirements for the iBind device.
Comparison of manually processed midi blots vs. midi blots processed with the iBind Flex Western System.
Samples containing GST-tagged recombinant proteins were separated and then transferred to nitrocellulose membranes using the Invitrogen iBlot 2 Dry Blotting System. Blots were probed with identical concentrations of the same pair of primary and secondary antibodies. The primary antibody was rabbit anti-GST diluted 1:500 (8 µL in 4 mL iBind Flex Solution for the iBind system, 40 µL in 20 mL for manual tray incubation). The secondary antibody was goat anti-rabbit HRP diluted 1:600 (6.7 µL in 4 mL iBind Flex Solution for the iBind system, 33.3 µL in 20 mL for manual tray incubation). For final detection, blots were incubated for 5 minutes in Thermo Scientific SuperSignal West Dura Substrate for visualization with an imaging system.
Comparison of manually processed mini blots vs. mini blots processed with the iBind Flex Western System.
Blots were produced by separating samples and then transferred to nitrocellulose membranes using the iBlot 2 Dry Blotting System. Each gel contained 10 lanes loaded with two-fold dilution series of 293 cell extracts (30 µg to 0.06 µg). After immunodetection using the conditions described below, blots were incubated for 5 minutes in Thermo Scientific SuperSignal West Dura Substrate and the signal documented with an imager.
Comparison of 6 manually processed vertically cut blot strips vs. strips processed with the iBind Flex Western System.
Process up to 6 strips with 6 different antibodies combinations at a single time. Blots were produced by separating samples and transferring to nitrocellulose membranes using the iBlot 2 Dry Blotting System.
Our starter kits include everything you need to start processing your western blots using the iBind Western Systems.
By contrast, iBind Western Systems allow all solutions to be prepared and loaded in the device at the start of the procedure, from which point all steps proceed automatically and uninterrupted by sequential lateral flow technology (SLF), i.e., simple capillary action—no electricity or batteries are required.
Western processing using the Invitrogen iBind and iBind Flex Western Systems require only a 15- to 30-minute setup time and no additional hands-on steps before you can move to final detection.
For Research Use Only. Not for use in diagnostic procedures.