Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
StemPro Accutase is a marine-origin enzyme with proteolytic and collagenolytic activity for the detachment of primary and stem cell lines and tissues.
StemPro Accutase allows single cell passaging of hESC when cultured in StemPro hESC SFM (Figure 1).
Figure 1. Robust single cell passaging of hESCs. BGO2 cells were disaggregated to single cells with StemPro Accutase reagent and plated in StemPro hESC SFM on a Geltrex substrate. Images taken 1.5 and 20 hours after plating indicate that cells migrate to form micro colonies. These cultures expand to form monolayers of undifferentiated cells after 5–6 days of growth.
StemPro Accutase allows efficient dissociation of hNSCs cultured in adherent and neurosphere suspension cultures (Figure 2).
![]() Panel A | ![]() Panel B | Figure 2. Dissociation of human neurosphere. Human neurosphere (A) dissociated into single cell culture (B) using 1 ml of StemPro Accutase reagent for 10 minutes. |
For Research Use Only. Not for use in diagnostic procedures.