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Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
1,8-ANS | 372 | 480 | ![]() | |
4-methylumbelliferone | 360 | 440 | ![]() | |
7-amino-4-methylcoumarin (AMC) | 351 | 430 | ![]() | |
7-hydroxy-4-methylcoumarin | 360 | 449 | ![]() | |
ABQ | 344 | 445 | ![]() | |
Acridine | 362 | 462 | ![]() | |
Alexa Fluor 350 | 346 | 442 | ![]() | |
Alexa Fluor 405 | 401 | 421 | ![]() | |
AMCA | 345 | 425 | ![]() | |
AMCA-X | 353 | 442 | ![]() | |
BFP | 382 | 448 | ![]() | |
CellTrace Calcein Blue | 373 | 440 | ![]() | |
CellTrace Calcein Violet | 401 | 451 | ![]() | |
Calcofluor White | 350 | 440 | ![]() | |
Cascade Blue | 377 | 420 | ![]() | |
Dansyl | 335 | 525 | ![]() | |
DAPI | 345 | 455 | ![]() | |
DiFMU (pH 9.0) | 357 | 450 | ![]() | |
DyLight 350 | 353 | 432 | ![]() | |
ER-Tracker Blue-White DPX | 371 | 554 | ![]() | |
Fast Blue | 360 | 440 | ![]() | |
FluoSpheres Blue | 356 | 412 | ![]() | |
Hoechst 33258 | 345 | 487 | ![]() | |
Hoechst 33342 | 347 | 483 | ![]() | |
LIVE/DEAD Fixable Blue Dead Cell Stain | 344 | 442 | ![]() | |
LysoSensor Blue | 374 | 424 | ![]() | |
LysoTracker Blue | 373 | 422 | ![]() | |
Marina Blue | 365 | 460 | ![]() | |
Methoxycoumarin | 360 | 410 | ![]() | |
Monochlorobimane(mBCI) | 380 | 461 | ![]() | |
NucBlue Fixed Cell Stain | 345 | 455 | ![]() | |
NucBlue Live Cell Stain | 347 | 483 | ![]() | |
Pacific Blue | 405 | 455 | ![]() | |
PBFI | 360 | 420 | ![]() | |
Phorwite AR | 360 | 430 | ![]() | |
Quinine Sulfate | 349 | 461 | ![]() | |
sgBFP (Super Glow BFP) | 387 | 450 | ![]() | |
SITS | 365 | 460 | ![]() | |
SpectrumBlue | 405 | 449 | ![]() | |
Thioflavin TCN | 350 | 460 | ![]() | |
Thiolyte | 378 | 483 | ![]() | |
True Blue | 365 | 425 | ![]() | |
Uvitex SFC | 365 | 435 | ![]() | |
Y66H | 360 | 442 | ![]() |
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor 405 | 401 | 421 | ![]() | |
DyLight 405 | 400 | 420 | ![]() | |
BFP | 382 | 448 | ![]() | |
Cascade Blue | 400 | 420 | ![]() | |
Pacific Blue | 410 | 455 | ![]() | |
SpectrumBlue | 400 | 450 | ![]() | |
TagBFP | 402 | 457 | ![]() |
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Auramine O | 445 | 500 | ![]() | |
CellLight CFP | 434 | 477 | ![]() | |
ECFP | 434 | 477 | ![]() | |
Evans Blue | 460 | 515 | ![]() | |
Lucifer yellow | 428 | 544 | ![]() |
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
5-carboxyfluorescein(5-FAM) | 492 | 518 | ![]() | |
Acridine Orange + DNA | 500 | 526 | ![]() | |
ActinGreen (Alexa Fluor 488 phalloidin) | 496 | 518 | ![]() | |
Alexa Fluor 430 | 432 | 540 | ![]() | |
Alexa Fluor 488 | 496 | 518 | ![]() | |
ATTO 465 | 453 | 507 | ![]() | |
Auramine | 460 | 540 | ![]() | |
Aurophosphine | 470 | 540 | ![]() | |
BCECF | 499 | 540 | ![]() | |
BODIPY 492/515 | 490 | 540 | ![]() | |
BODIPY 505/515 | 502 | 540 | ![]() | |
BODIPY FL | 502 | 540 | ![]() | |
BTC | 464 | 540 | ![]() | |
Calcein | 494 | 540 | ![]() | |
Calcium Green-1 | 506 | 540 | ![]() | |
Catskill Green 540 | 482 | 540 | ![]() | |
CellLight GFP | 488 | 507 | ![]() | |
CellMask Green Actin Tracking Stain | 503 | 512 | ![]() | |
CellEvent Caspase-3/7 Green | 488 | 507 | ![]() | |
CellTracker Green | 490 | 540 | ![]() | |
CellRox Green | 485 | 520 | ![]() | |
CFDA | 494 | 540 | ![]() | |
CFP | 434 | 540 | ![]() | |
Click-iT EdU Alexa Fluor 488 | 496 | 518 | ![]() | |
Cy2 | 492 | 540 | ![]() | |
CyQUANT Direct (CyQUANT GR) | 500 | 540 | ![]() | |
DAF-FM | 493 | 540 | ![]() | |
DiO (Vybrant DiO) | 489 | 506 | ![]() | |
Diversa Green-FP | 484 | 507 | ![]() | |
DyLight+C110 488 | 493 | 518 | ![]() | |
Emerald Green | 490 | 540 | ![]() | |
Evans Blue | 460 | 515 | ![]() | |
FITC (Fluorescein) | 492 | 520 | ![]() | |
Fluo-3 | 506 | 540 | ![]() | |
Fluo-4 | 494 | 540 | ![]() | |
Fluoro-Emerald | 494 | 518 | ![]() | |
Fluoro-Jade B | 480 | 525 | ![]() | |
Fluor-X | 494 | 520 | ![]() | |
FluoSpheres Yellow-Green | 501 | 513 | ![]() | |
GFP (EGFP) | 488 | 507 | ![]() | |
H2DCFDA (H2-DCF,DCFR) | 504 | 540 | ![]() | |
HCS LipidTox Green | 498 | 506 | ![]() | |
HPTS (Solvent Green 7) | 455 | 510 | ![]() | |
LIVE/DEAD Fixable Green | 498 | 526 | ![]() | |
Lucifer Yellow | 423 | 543 | ![]() | |
LysoSensor Green | 448 | 503 | ![]() | |
LysoTracker Green | 504 | 511 | ![]() | |
Magnesium Green | 506 | 530 | ![]() | |
mHoneydew | 478 | 561 | ![]() | |
MitoTracker Green FM | 490 | 512 | ![]() | |
NBD Amine | 450 | 530 | ![]() | |
NBD-X | 467 | 538 | ![]() | |
NeuroTrace 500/525 Green | 497 | 524 | ![]() | |
Nitrobenzoxadiazole | 465 | 510 | ![]() | |
NucGreen Dead Cell Stain | 504 | 523 | ![]() | |
Oregon Green 488 | 498 | 524 | ![]() | |
Oregon Green 514 | 512 | 532 | ![]() | |
pHrodo Green | 509 | 533 | ![]() | |
Premo FUCCI Cell Cycle Sensor (S/G2/M phases) | 474 | 509 | ![]() | |
Qdot 525 | UV | 525 | ![]() | |
Qdot 545 | UV | 543 | ![]() | |
Quant-iT PicoGreen | 502 | 522 | ![]() | |
Rhodamine 110 | 496 | 520 | ![]() | |
Rhodamine 123 | 507 | 529 | ![]() | |
Rhodamine Green | 497 | 523 | ![]() | |
Rhodol Green | 496 | 523 | ![]() | |
rsGFP (red shifted GFP, S65T) | 498 | 516 | ![]() | |
S65C | 479 | 507 | ![]() | |
S65L | 484 | 510 | ![]() | |
S65T | 488 | 511 | ![]() | |
sgGFP (Super Glow GFP) | 474 | 509 | ![]() | |
Sodium Green | 506 | 532 | ![]() | |
SpectrumGreen | 498 | 522 | ![]() | |
Surf Green EX | 469 | 526 | ![]() | |
SYBR Gold | 495 | 539 | ![]() | |
SYBR Green I | 497 | 521 | ![]() | |
SYBR Safe | 507 | 524 | ![]() | |
SYTO 9 | 482 | 500 | ![]() | |
SYTO 11 | 506 | 525 | ![]() | |
SYTO 13 | 487 | 505 | ![]() | |
SYTO 16 | 490 | 520 | ![]() | |
SYTO 45 | 452 | 484 | ![]() | |
SYTO RNASelect | 503 | 527 | ![]() | |
SYTOX Green | 504 | 523 | ![]() | |
TET | 520 | 541 | ![]() | |
TO-PRO-1 | 515 | 530 | ![]() | |
TOTO-1 | 513 | 531 | ![]() | |
Vybrant DyeCycle Green | 505 | 532 | ![]() | |
wtGFP (wild type GFP, non-UV excitation) | 475 | 509 | ![]() | |
YFP | 512 | 529 | ![]() | |
YO-PRO-1 | 491 | 507 | ![]() | |
YOYO-1 | 491 | 509 | ![]() |
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Acridine Orange + DNA | 500 | 526 | ![]() | |
EYFP | 514 | 527 | ![]() |
*See the complete list of CellLights Reagents
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor 660 | 663 | 691 | ![]() | |
Alexa Fluor 680 | 679 | 702 | ![]() | |
Alexa Fluor 700 | 696 | 719 | ![]() | |
ATTO 635 | 635 | 658 | ![]() | |
ATTO 647 | 644 | 670 | ![]() | |
ATTO 655 | 663 | 683 | ![]() | |
ATTO 680 | 680 | 700 | ![]() | |
ATTO 700 | 699 | 719 | ![]() | |
Cy5.5 | 672 | 690 | ![]() | |
DY-675 | 675 | 699 | ![]() | |
DyLight 680 | 676 | 705 | ![]() | |
Fast Blue | 615-672 | 730-790 | ![]() | |
FluoSpheres Dark Red fluorescent microspheres | 656 | 683 | ![]() | |
HiLyte Fluor 680 | 688 | 702 | ![]() | |
IRDye 680 NHS Ester | 683 | 710 | ![]() | |
IRDye 700 Phosphoramidite | 691 | 699 | ![]() | |
Qdot 705 Nanocrystals | 300 | 702 | ![]() | |
Quasa 705 | 688 | 706 | ![]() | |
SYTO 60 | 650 | 681 | ![]() |
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Alexa Fluor Plus 750 Phalloidin | 758 | 784 | ![]() | |
CellVue NIR780 | 743 | 776 | ![]() | |
Cy7 | 745 | 766 | ![]() | |
DyLight 750 | 752 | 778 | ![]() | |
Indocyanine Green | 768 | 807 | ![]() | |
IRDye 800CW | 774 | 789 | ![]() | |
LIVE/DEAD Fixable near-IR | 750 | 775 | ![]() | |
XenoLight CF770 | 770 | 797 | ![]() | |
HCS CellMask Near-IR Stain | 777 | 794 | ![]() |
Note: Near Infrared dyes (e.g., IRDye 800CW) work best with low-magnification, high-NA objectives to maximize detection sensitivity.
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
CFP | 434 | 477 | Control YFP Emission |
Notes: CFP-YFP FRET control. CFP excitation coupled with a YFP emission filter. For FRET sensitized-emission donor-acceptor bleedthrough measurements. Requires CFP and YFP light cubes for CFP-YFP FRET experiments.
Dye | Abs Peak | Em Peak | Spectral Match | |
---|---|---|---|---|
Acridine Orange + RNA | 460 | 650 | ![]() | |
Acridine Orange + DNA | 500 | 526 | ![]() | |
Alexa Fluor 430 | 434 | 540 | ![]() | |
AmCyan | 548 | 489 | ![]() | |
Auramine O | 460 | 550 | ![]() | |
CTC Formazan | 450 | 630 | ![]() | |
Di-8 ANEPPS | 468 | 635 | ![]() | |
DiA (FAST DiA) | 491 | 613 | ![]() | |
Lucifer Yellow | 428 | 544 | ![]() | |
LysoSensor Green (pH 5) | 442 | 505 | ![]() | |
PerCP | 488 | 675 | ![]() |
Notes: Acridine Orange, longpass emission. To visualize Acridine Orange in green and orange/red emission wavelengths, we recommend using this light cube with the EVOS FL color or EVOS FL Auto cell imaging systems. The AO cube passes simultaneous green, yellow, and red fluorescence, which requires color camera detection. Works well with other fluorophores listed below in either monochrome or color camera EVOS FL cell imaging systems, provided the sample is labeled with only one dye.
For Research Use Only. Not for use in diagnostic procedures.