Thermo Scientific phosphoramidites with ribonucleobases for RNA oligo synthesis are built to meet the varying requirements of oligonucleotide manufacturers from the proof of concept of research application (research use only, RUO) and development (R&D) to the process development of therapeutic and diagnostic applications. Backed by documentations and certificate of analysis, choose from multiple quality grades of standard, TheraPure, and TheraPure with fast deprotect phosphoramidites that best fit your needs.

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TheraPure RNA phosphoramidites

Premium-grade and high-purity phosphoramidites are manufactured under extreme quality control and QC testing before product release. Ideal for any therapeutics application with excellent batch-to-batch reproducibility.

Availability by synthesizer compatibility

 rUiBu-rGAc-rCBz-rA
Applied Biosystems/AKTA Synthesizer27-1804-0227-1906-02 27-1805-0227-1903-02
Expedite Synthesizer27-1804-1027-1906-1027-1805-1027-1903-10
MerMade Synthesizer27-1804-1327-1906-1327-1805-1327-1903-13
Bulk (None) Synthesizer27-1804-5027-1906-5027-1805-5027-1903-50

Technical specifications of TheraPure RNA phosphoramidites

Extensive QC testing to help ensure impurities and residual solvents are within the stringent requirement for oligo therapeutics.

  Click images to enlarge

 Bz-rA CEPiBu-rG CEPAc-rC CEPrU CEP
Cat. No. 27-190327-190627-180527-1804
FormulaC53H66N7O8PSiC50H68N7O9PSiC47H64N5O9PSiC45H61N4O9PSi
Molecular weight

988.2 g/mol970.1 g/mol902.1 g/mol861.1 g/mol
CAS #104992-55-4147201-04-5121058-88-6118362-03-1
AppearanceColor intensity must not exceed Pantone #P1-4U or #P4-2U
Solution clarity  Free from undissolved particulate at 0.2 M solution in ACN
HPLC purity≥99%
31P NMR purity≥99%
Residual solvent≤3.0% total % by weight solvent residue
Shipping / Storage conditionAmbient / –20°C
Water content ≤0.3%
Material originNon-animal source (no TSE/BSE risk)

Compatible products by synthesizer model

Available TheraPure RNA phosphoramidites are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer
  • Bulk

Applied Biosystems and AKTA synthesizer

Compatible products are in amber bottles with serum vial neck style as ready-to-ship catalog SKU.

Expedite synthesizer

Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style, which is compatible with the Expedite synthesizer for oligo synthesis.

MerMade systems synthesizer

Catalog SKUs available in 30 mL amber bottle with a 28-400 finish neck style are compatible with the MerMade synthesizer for oligo synthesis.

Bulk synthesizer

Compatible catalog SKUs are offered in great value pack. Supplied in 250 mL opaque bottle with a wide mouth neck style, which is compatible with most bulk oligonucleotide synthesizers for oligo synthesis.

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TheraPure RNA phosphoramidites with fast protect

Fast deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents and are available in premium TheraPure grade. Excellent for applications requiring mild deprotection to preserve the integrity of oligonucleotides.

Availability by synthesizer compatibility

 PAC-rAiPrPAC-rG
Applied Biosystems/AKTA Synthesizer27-1803-0227-1806-02
Expedite Synthesizer27-1803-0927-1806-09

Technical specifications for fast deprotect RNA phosphoramidites

Rigorous QC testing is done to help ensure high purity with the removal of base-protecting groups allowed without needing special reagents. 

  Click images to enlarge

 TheraPure PAC-rA CEP *TheraPure iPrPAC-rG CEP *TheraPure Ac-rG CEP **
Cat. No.27-180327-1806SY3000
FormulaC54H68N7O9PSiC57H74N7O10PSiC48H64N7O9PSi
Molecular weight1018.2 g/mol1076.3 g/mol942.14 g/mol
CAS #121058-86-4956580-73-7944138-03-8
Solution clarity0.2 M solution in ACN is free from undissolved particulate
HPLC purity≥99%
31P NMR purity≥99%
Water content ≤0.3%
Shipping / Storage conditionAmbient / –20°C
Material origin Non-animal source (no TSE/BSE risk)
942.14 g/mol
942.14 g/mol

* When using both of PAC-rA (Cat No. 27-1803) and iPrPAC-rG (Cat No. 27-1806), select Ac-rC (Cat No. 27-1805) and rU (Cat No. 27-1804) to minimize the time for post-synthesis deprotection.

** The acetyl-protected rG (Ac-rG, Cat No. SY3000) amidite is a suitable replacement for iBu-rG (Cat No. 2706) and is ideal for synthesis of longmers. Oligonucleotide deprotection times can be reduced as much as 25%. 

Compatible products by synthesizer model

Available TheraPure RNA phosphoramidites with fast deprotect features are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer

Applied Biosystems and AKTA synthesizer

Compatible products are in amber bottles with serum vial neck style as ready-to-ship catalog SKU.

Expedite synthesizer

Ready-to-ship in 30 mL amber bottle with a 20-400 finish neck style for oligo synthesis.

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Thermo Fisher Standard RNA phosphoramidites

Traditional base protection methods to help ensure quality and reliability of routine oligonucleotide synthesis.

Availability by synthesizer compatibility

 rUiBu-rGAc-rCBz-rA
Applied Biosystems/AKTA Synthesizer27-1404-0727-1406-0727-1405-0727-1403-07

Technical specifications for standard RNA phosphoramidites

  Click images to enlarge

 Bz-rA CEPiBu-rG CEPAc-rC CEPrU CEP
Cat. No.27-140327-140627-140527-1404
FormulaC53H66N7O8PSiC50H68N7O9PSiC47H64N5O9PSiC45H61N4O9PSi
Molecular weight

988.2 g/mol970.1 g/mol902.1 g/mol861.1 g/mol
CAS #104992-55-4147201-04-5121058-88-6118362-03-1
AppearanceColor intensity must not exceed Pantone #P1-4U or #P4-2U
HPLC purity≥98.0%
31P NMR purity ≥98.0%
Shipping / Storage conditionAmbient / –20°C
Water content ≤0.3%
Material originNon-animal source (no TSE/BSE risk)

Compatible products with the Applied Biosystems and AKTA synthesizer

Available and ready-to-ship in amber bottles with serum vial neck style for RNA oligo synthesis.

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Quality manufacturing and analytical method

Manufacturing quality

HPLC-MS, GC, 31P NMR, and Karl Fischer oven titration are used to assess the purity of each phosphoramidite lot. The rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities and accumulation of by-products during oligo synthesis. Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility under the ISO 9001:2015–certified quality management system.

Analytical method and data

Phosphoramidites are the key raw material and building blocks of oligonucleotide therapeutics. Incorporating the quality testing step into the manufacturing process development from the very beginning is a best practice needed for regulatory documentation. 

Example: TheraPure Bz-dA phosphoramidite

For the successful synthesis of a therapeutic from discovery to market, a thorough understanding on the analysis of phosphoramidites and impurity is crucial. Here is information on the analytical method used and the details on the instrument conditions used to test the quality of the raw material phosphoramidites.

UHPLC analysis method

Condition setup
UHPLC systemThermo Scientific Vanquish UHPLC System
DetectionThermo Scientific Vanquish Diode
Array Detector, 255 nm; bandwidth: 5 nm; ref: off
ColumnWalters ACQUITY UPLC Oligonucleotide BEH C18 Column,
2.0 x 100 mm, 1.7 µm (Cat. No. 186003950))
Column temperature25°C
Sample temperature4°C
Injection volume3.0 µL
Mobile phase A50% 10 mM ammonium acetate (pH 9.0), 50% acetonitrile
Mobile phase B5% 10 mM ammonium acetate (pH 9.0), 95% acetonitrile
Sample preparation0.5 mg/mL in acetonitrile dried over molecular sieves
Gradient
Time (min)Flow (mL/min)A (%)B (%)
00.25545
1.50.25545
170.2298
300.2298


Figure 1. Impurity profile of TheraPure iBu-rG phosphoramidite from UHPLC chromatograms

  • Full chemical name: 5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-O-[(tert-butyl)dimethylsilyl]-guanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • Base: rG; Base protection: iBu
  • HPLC purity: ≥99%; M-11 Impurity: ≤0.01%
  • 31P NMR purity: ≥99%; Sum of non-primary peaks in 140–152 ppm range: ≤0.5%; No single PIII impurity >0.3%

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Resources

Phosphoramidite Learning Resource

Access webinars, videos, articles, and more to optimize your success in oligonucleotide synthesis for diagnostic and therapeutic manufacturing process development.

mRNA Production Learning Library

Browse the educational assets related to raw materials selection, process development using in vitro transcription, scale-up synthesis, and the latest trends in mRNA production.

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For Research Use Only. Not for use in diagnostic procedures.