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The Applied Biosystems KaryoStat and KaryoStat HD Assays provide an alternative to g-banding karyotyping for pluripotent stem cell lines. The assays offer accurate genotyping (sample ID) plus whole-genome coverage for accurate detection of chromosomal abnormalities with results in 3 to 4 days. The assay includes arrays, reagents, and easy-to-use data analysis software for a cost-effective and streamlined analysis of pluripotent stem cell lines. Now researchers won't have to rely on g-banding karyotype service providers.
KaryoStat and KaryoStat HD Arrays are compatible with GeneChip Scanner 3000.
The KaryoStat and KaryoStat HD Assays include access to free downloadable Chromosomal Analysis Suite (ChAS) Software that allows researchers to confirm absence of chromosomal abnormalities in PSC lines.
You can leverage our KaryoStat Karyotyping Services for help in assaying genomic integrity in your cell cultures.
KaryoStat Assay has a resolution similar to g-banding karyotyping and is recommended for most stem cell research applications. The KaryoStat HD Assay provides higher resolution analysis with greater than 99% sensitivity and can reliably detect 25–50 kb copy number changes across the genome at high specificity with single-nucleotide polymorphism (SNP) allelic corroboration.
KaryoStat Assay | KaryoStat HD Assay | |
---|---|---|
Gains | >2 Mb | >25–50 kb |
Losses | >1 Mb | >25–50 kb |
Absence of heterozygosity | >5 Mb | >1 Mb |
SNP | Yes | Yes |
Coverage | Low | Highest |
Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.