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Bovine pulmonary artery endothelial (BPAE) cells or RAW macrophage cells were plated in 96 well plates. BPAE cells were treated with or without 100 µM menadione for 1 hr. 100 µM of superoxide scavenger, MnTBAP was added to some of the control and menadione-treated wells for the last 30 mins of incubation. RAW cells were treated with or without 500 ng/ml of LPS +/- 150 nM diphenyleneiodonium (DPI), a NADPH oxidase inhibitor. The cells were then stained with 5 µM Far Red ROS Sensor by adding the probe to the complete media. The cells were then washed with PBS and analyzed on a Thermo Fisher Cellomics ArrayScan® VTI. MnTBAP or DPI treatment inhibited ROS caused by menadione or LPS, respectively, confirming that the signal was due to ROS induced by these compounds. ***a The values are significantly different from controls with P = 0.0001 The values are significantly different from controls with P = 0.0001; ***b values were signficantly different from drug treated cells with P = 0.0001
BPAE detection of oxidative stress using MitoTracker® Green (Cat. No. M7514) and CellROX® Deep Red Reagent (Cat. No. C10422) Go ›
BPAE imaging using CellROX® Deep Red Reagent (Cat. No. C10422) Go ›
Live cell imaging with CellLight™ reagents. Go ›
Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›
CD335 (NKp46) Antibody (63335182) in RE Go ›
CD223 (LAG-3) Antibody (56223942) in TM Go ›