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VeriFiler PCR Amplification Kits
VeriFiler Plus and VeriFiler Express PCR Amplification kits are designed to meet challenging forensic casework and databasing needs globally. Utilizing our 6-dye STR chemistry, the VeriFiler kits contain a total of 25 markers—23 autosomal STRs, including Penta D and Penta E, and two gender discrimination markers. The kits are manufactured in our ISO18385 certified facility and are supported by our comprehensive training, service, and applications teams.
With 11 mini-STRs, VeriFiler Plus kits are specifically designed for challenging sample types: touch, inhibited, or degraded samples, with improved sensitivity and robustness to inhibition. In addition, an Internal Quality Control (IQC) system assesses for the presence of inhibitors in the sample to enable distinction between degraded and inhibited samples which can help forensics analysts make better decisions on how to further process problematic samples. The IQC system also verifies PCR amplification. Although optimized for casework samples, direct amplification of single-source reference samples using the VeriFiler Plus kit is also supported to enable laboratories to process all sample types with one amplification kit.
VeriFiler Express kits offer superior discrimination power and high throughput, with efficient processing from single source samples to profile in as little as two hours. They have increased flexibility for use with multiple sample collection devices and there are protocols available for the most commonly used substrates.
The VeriFiler Plus and VeriFiler Express kits are approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.
Allele size comparison for VeriFiler Plus kit (left) and VeriFiler Express kit (right)
Click image to enlarge
Click image to enlarge
| VeriFiler Plus kit | Verifiler Express kit |
High discrimination power | 25-marker multiplex assay that includes two gender markers as well as the highly discriminatory Penta D and Penta E markers | 25-marker multiplex assay that includes two gender markers as well as the highly discriminatory Penta D and Penta E markers |
Improved sensitivity | Kit allows for loading up to 17.5 µL of sample volume per 25 µL reaction | - |
Optimized chemistry |
| Validated with multiple sample collection devices such as treated paper, untreated paper, and swabs; designed to work with the most commonly used substrates |
Internal Quality Control system | Yes: for distinguishing inhibited and degraded samples; positive control for PCR amplification | No |
DNA input |
|
|
NDIS approved | Yes | Yes |
Featured publications
Developmental validation of VeriFiler Plus PCR Amplification Kit: A 6-dye multiplex assay designed for casework samples Green, Robert et al.Forensic Science International: Genetics, Volume 53, 102494
Sensitivity and baseline noise of three new generation forensic autosomal STR kits: PowerPlex Fusion, VeriFiler Plus and Investigator 24plex QS. Tay, Jasmine et al. Forensic ScienceInternational: Reports,Volume 1,2019,100049.
Need help bringing products online?
Look no further than our Human Identification Professional Services (HPS) team. Since 2007, we have completed over 400 successful validation projects worldwide with a team of more than 20 technical support specialists, each averaging eight years of real-world forensic experience, providing customers with in-depth training and support on our instruments, chemistries, and software.
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- Call: 1-888-821-4HID (4443), select Option #1
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If you have additional comments or questions, please contact the customer service team via email.
For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.
For licensing and limited use restrictions visit thermofisher.com/HIDlicensing