Microarray Labeling Kits and Components for Array Genomic Profiling

More Correct Calls With Higher Signal-to-Noise Ratios

Life Technologies BioPrime® labeling systems enable reliable and consistent genomic DNA labeling and efficient removal of free dye and nucleotides. And because results obtained using these reagents exhibit improved signal-to-background and reduced channel bias, you can count on greater accuracy.

	Sample Type
Platform	gDNA	ChiP*	FFPE	DNA Input Amount	Labeling Method	Label	Master Mix	Product
Non-Platform Specific				5 ng–3 μg	Direct	AlexaFluor® 3 AlexaFluor® 5 (included)		BioPrime® Total Genomic Labeling System 18097011 - (30 reactions, w/ purification) 18097012 - (30 reactions, w/o purification) 18097010 - (10 reactions, w/ purification)
				250 ng–1 μg (Bac arrays) 4 μg (cDNA arrays)	Direct	User’s choice (not included)		BioPrime® aCGH Genomic Labeling System 18095011 - (30 reactions, w/ purification) 18095012 - (30 reactions, w/o purification)
				Array-dependent	Direct	AlexaFluor® 3 AlexaFluor® 5 (included)		BioPrime® Total FFPE Genomic Labeling System A10965011 - (30 reactions, w/ purification)
* ChIP must first be performed using a kit such as the MAGnify™ Chromatin Immunoprecipitation System, Cat. No. 492024

Repetitive Sequence Blocking Reagents

Product	Size	Cat. No.
Human Cot-1 DNA®-Fluorometric QC	1 mg	15729101
Human Cot-1 DNA®-Fluorometric QC	500 μg	15279011
Mouse Cot-1 DNA	500 μg	18440016
Yeast tRNA	25 mg	15401011

Which BioPrime® system is right for me?

The BioPrime® Total aCGH reagents offer various configurations to allow for optimal performance on arrays based on premier enzymes, primers, dyes, and purification systems. For two-color array CGH and ChIP-on-chip experiments, we recommend the BioPrime® Total Genomic Labeling system. The BioPrime® Total Genomic Labeling system allows for the lowest starting input material, includes application-optimized dye-labeled nucleotides (with Alexa Fluor® 3 and Alexa Fluor® 5 dyes), and is the simplest, easiest-to-use kit in the product family. For researchers who wish to use other dyes, we offer BioPrime® aCGH Genomic Labeling systems without dyes—this allows you to take advantage of the higher-performance enzymes and reagents in our BioPrime® kits to deliver superior yield and better total dye incorporation.