Mycotoxin and Biotoxin Analysis

With simple, fast sample preparation, this economical and robust method can be used for the quantitation of all 11 legislated mycotoxins worldwide and 37 more that are either already legislated in feed or are prospects for further legislation. The lowest maximum residue levels (MRLs) were reached for all regulated compounds with good precision and reproducibility on five replicates at the limit of detection.

This robust method uses a simple liquid-liquid extraction and a dilute-and-shoot approach to achieve high analyte recovery and reliable quantitation. The method was fully validated in the laboratory four analytical runs with duplicate calibration curves along with 5 quality control sample replicates at low, medium, and high concentrations. The method produced excellent % RSDs for the target analytes over the course of the analytical runs.

This method offers an excellent solution for the rapid screening and quantitation of multiple mycotoxin compounds in a single method. The simple QuEChERS extraction procedure from a corn-based animal feed matrix provides an efficient and robust workflow with no further cleanup. All three scan modes studied showed excellent quantitation performance with MS² fragment matching and library searching against highly curated databases.

Fast polarity switching was very effective, with mass accuracies remaining stable across the peak in both polarities, providing plenty of scans across the peak for accurate quantitation. The ability to analyze a sample extract in both positive and negative ion modes, in a single analytical run instead of two, offers a huge productivity advantage.

To help you deliver high quality identification of mycotoxins, we regularly curate our compound and spectral database library for natural toxins to reduce your database development time and cost, while ensuring ultimate confidence and confirmation in the data you report. Though some libraries may have more compounds, these are based on estimated structures developed on lower resolution QTOF instrumentation – lower quality data will give more errors and lower confidence in library searches.

Confirmation of target mycotoxins was performed using MS² fragment matching and searches against the Thermo Scientific mzCloud highly curated MS² and MSⁿ data mass spectral database library.

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QuEChERS Dispersive SPE products

• Complete kits provide a quick, rugged, and cost-effective process for the extraction of multiple mycotoxin analytes.
• Higher sample recovery and throughput, reduced labor, and lower reagent and solvent costs enable better productivity and lower costs per sample.