Transfection Reagents - Product Protocols

Lipofectamine 3000

Lipofectamine 3000 Transfection Reagent:
Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable superior transfection performance and reproducible results. It delivers exceptional transfection efficiency into the widest range of difficult-to-transfect and common cell types with improved cell viability

Lipofectamine Stem

Lipofectamine Stem Transfection Reagent:
Lipofectamine Stem Transfection Reagent is optimized to achieve maximum efficiency with minimal early differentiation in a wide range of stem cells. Designed for co-delivery of DNA, RNA, and Cas9 ribonucleoprotein (RNP) complexes, Lipofectamine Stem reagent is compatible with a variety of media systems, including feeder-free, helping support and simplify your stem cell culture workflow. Researchers can achieve up to 80% or better transfection efficiency in pluripotent stem cells (PSCs) and neural stem cells (NSCs), and up to 45% in meschenchymal stem cells (MSCs). See also cell specific protocols.

Lipofectamine 2000

Lipofectamine 2000 Transfection Reagent:
A proprietary cationic lipid formulation that offers the highest transfection efficiencies and protein expression levels in a wide variety of adherent and suspension cell lines

Cotransfecting RNAi and DNA with Lipofectamine 2000:
This reference provides general guidelines and a procedure to co-transfect plasmid DNA and an RNAi molecule (i.e. Stealth RNAi, siRNA, shRNA plasmid or miRNA plasmid) into mammalian cells using Lipofectamine 2000 Reagent

Lipofectamine 2000 Reverse Transfection:
Reverse transfection combines RNA, transfection reagent, and cells in an altered sequence compared to traditional Lipofectamine 2000 transfection protocols; in short, a different RNAi molecule is put in each well prior to transfection and combined with diluted Lipofectamine 2000 to form complexes in each well; cells are added directly to the Lipofectamine 2000-RNA complexes and transfection occurs while cells are attaching to the well

Lipofectamine LTX Reagent

Lipofectamine LTX:
An early generation reagent formulated for the transfection of DNA into eukaryotic cells

Lipofectamine LTX Reagent Cell-type Specific Protocols:
These protocols provide a recommended procedure to transfect plasmid DNA into different types of cells

Transfecting Plasmid DNA into Primary Mouse Neural Progenitor Cells Using Lipofectamine LTX Reagent:
This reference provides a customer-generated protocol for transfecting plasmid DNA into primary mouse neural progenitor cells. Please note that this protocol has not been verified by scientists at Invitrogen. It is provided as references of transfection in challenging cell type to share with all customers. Further optimization of this protocol might be necessary in individual laboratories

Transfecting Plasmid DNA into RAW264.7 Macrophage Cells Using Lipofectamine LTX Reagent:
This reference provides a customer-generated protocol for transfecting plasmid DNA into Raw 264.7 Macrophage cells. Please note that this protocol has not been verified by scientists at Invitrogen. It is provided as references of transfection in challenging cell type to share with all customers. Further optimization of this protocol might be necessary in individual laboratories

Lipofectamine MessengerMAX

Lipofectamine MessengerMAX Transfection Reagent:
A Transfection Reagent that delivers amazing transfection efficiency in neurons and a broad-spectrum of primary cells, enabling improved application outcomes and more biologically relevant research

Lipofectamine RNAiMAX

Lipofectamine RNAiMAX Transfection Reagent:
A proprietary RNAi-specific cationic lipid formulation that offers the highest transfection efficiencies on the widest variety of cell types for siRNA gene knockdown experiments

Lipofectamine RNAiMAX Transfection Reagent Cell-type Specific Protocols:
These protocols provide a recommended procedure to transfect siRNA into different types of cells

Invivofectamine 3.0

Invivofectamine 3.0 Reagent:
Description: A breakthrough reagent for in vivo RNAi delivery, with greatly improved performance and up to 85% knockdown achieved using microgram levels of siRNA

Other reagents

FreeStyle MAX Reagent:
A breakthrough technology for rapid and high-yield mammalian protein production in suspension - adapted CHO and 293 cells.

293fectin Transfection Reagent:
A specially designed formulation which offers extremely high transfection efficiencies of FreeStyle 293-F suspension cells in serum-free GIBCO FreeStyle 293 Expression Medium

Oligofectamine Transfection Reagent:
Specially formulated for stable complexes with oligos, permitting efficient transfection into eukaryotic cells

Lipofectamine Transfection Reagent:
One of our first generation cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells

Lipofectin Transfection Reagent:
First generation transfection reagent for endothelial cells; some of our customer also use it for transfection of DNA, RNA or oligonucleotides into their cell line of choice

DMRIE-C Transfection Reagent:
A liposomal formulation which complexes with nucleic acids for efficient transfection into mammalian cells

Calcium Phosphate Transfection Kit:
An inorganic transfection reagent that allows for delivery of DNA into eukaryotic cells via calcium phosphate co-precipitation

Cellfectin II Transfection Reagent:
A cationic-lipid formulation designed for optimal transfection of insect cells