Proteases and Protein-Cleaving Reagents

We offer purified and agarose-immobilized proteases for enzymatic proteolysis (cleavage or digestion) of proteins to facilitate amino acid sequencing, peptide analysis, and polypeptide structural characterization.

For protein digestion using mass spectrometry, we offer a wide range of highly purified and MS-validated proteases.

Choose the right protease for your application

Fusion tag removal
EnzymePurified proteaseImmobilized proteasesSpecificity
Factor Xa32521
32520		N/ACleaves at the carboxyl side of Ile-Glu-Gly-Arg sequence
Human Rhinovirus 3C Protease
(HRV 3C)		88946N/AHighly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue
S. aureus V-8 Protease2019520151Cleaves at the carboxyl side of aspartate and glutamate residues
Antibody fragmentation
EnzymePurified proteaseImmobilized proteasesSpecificity
FicinN/A44881Cleaves non-specifically at exposed residues, favors aromatic residues
PapainN/A20341Cleaves nonspecifically at exposed residues
PepsinN/A20343Cleaves nonspecifically at exposed residues, favors aromatic residues
Proteomics using MS Analysis
EnzymePurified proteaseImmobilized proteasesSpecificity
Trypsin, MS Grade90057
90058
90059
90305		N/ACleaves at the carboxyl side of arginine and lysine residues
Lys-C Protease, MS Grade90051N/ACleaves at the carboxyl side of lysine residues
Trypsin/Lys-C Protease Mix, MS GradeA41007N/ACleaves at the carboxyl side of lysine residues
Lys-N Protease, MS Grade90300
90301		N/ACleaves at the amino side of lysine residues
Asp-N Protease, MS Grade90053N/ACleaves at the amino side of aspartate residues
Chymotrypsin, MS Grade90056N/ACleaves at the carboxyl side of tyrosine, phenylalanine, tryptophan, and leucine
Glu-C Protease, MS Grade90054N/ACleaves at the carboxyl side of glutamate or aspartate
Adherent cells dissociation
EnzymePurified proteaseImmobilized proteasesSpecificity
Trypsin (TPCK)2023320230Cleaves at the carboxyl side of arginine and lysine residues
DNase/RNase deactivation
EnzymePurified proteaseImmobilized proteasesSpecificity
Proteinase K17916N/ACleaves at the carboxyl side of aliphatic, aromatic, and hydrophobic residues

Bulk or custom product request

Need larger quantities of our protein labeling, crosslinking or modification reagents? We offer bulk and custom fill sizes.

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Resources

For Research Use Only. Not for use in diagnostic procedures.