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Pluripotent Stem Cell Resource Handbook
Your essential guide to stem cell research
Whether you’re new to the field or expanding into new areas, this educational handbook will provide you with everything you need for successful pluripotent stem cell (PSC) research. The PSC Resource Handbook offers the latest tools, resources, applications and more, to support every step of your PSC workflow.
Request a complete digital or printed copy of the handbook or access selected chapters using the links below.
Table of Contents
Part I – Reprogramming
- 1.1 Introduction
- 1.2 Choosing a reprogramming method
- 1.3 Reprogramming with episomal vectors
- 1.4 Reprogramming with Sendai virus (SeV)
Part II – Culture
- 2.1 Introduction
- 2.2 Feeder-dependent culture systems
- 2.3 Feeder-free culture systems
- 2.4 Choosing a culture system
- 2.5 Adapting to feeder-free culture systems
- 2.6 Cryopreservation
Part III – Transfection
- 3.1 Introduction
- 3.2 Choosing a transfection method and technologies
- 3.3 Transfecting DNA
- 3.4 Transfection with mRNA
- 3.5 Transfection with RNP complexes
Part IV – Genome editing
- 4.1 Introduction
- 4.2 Genome editing tools
- 4.3 CRISPR-Cas9 technology
- 4.4 TAL effector technology
- 4.5 Screening methods for TAL and CRISPR
Part V – Differentiation
- 5.1 Introduction
- 5.2 Neural differentiation
- 5.3 Cardiomyocyte differentiation
- 5.4 Definitive endoderm differentiation
Part VI – Characterization
- 6.1 Introduction
- 6.2 Detecting self-renewal marker expression
- 6.3 Evaluating differentiation potential
Part VII – Applications for cell therapy
- 7.1 Introduction
- 7.2 Translating research into clinical evaluation
- 7.3 Cell Therapy Systems products
- 7.4 Custom services and scalability
- 7.5 References
Part VIII – CellModel Services
Part IX – Gibco Stem Cell Training Courses
For Research Use Only. Not for use in diagnostic procedures.