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mRNA Testing and Vaccine Workflows
RNA analysis has been a key infectious disease research and testing. There are several ways for quantifying cellular RNA, each with its own set of benefits and drawbacks. One method may be better than the other depending on the nature of the experiment and the real scientific issues asked. Besides research and testing, RNA technology has also advanced into therapeutic applications.
Compared with traditional and recombinant protein vaccines, mRNA is transcribed and synthesized in vitro, which has obvious advantages in its development cycle and production cycle and has become a new hope for fighting against large-scale explosive infectious diseases. Thermo Fisher can provide an overall solution from identification, production, and purification to delivery of mRNAs. As a professional raw material supplier, Thermo Fisher can also provide complete OEM solutions for in vitro transcription to better meet customers’ needs for free of animal-origin ingredients and antibiotics, and a wider range of quality inspections.
Thermo Scientific™ KingFisher™ Flex Automated Nucleic Acid Extraction Instrument
The Thermo Scientific™ KingFisher™ Flex Purification System provides highly versatile and reproducible purification of 24 or 96 samples per run. It can be used with a variety of reagents, including Dynabeads products or MagMAX nucleic acid extraction kits, enabling scientists to process samples for a variety of applications.
Applied Biosystems™ MagMAX™ Virus/Pathogen Nucleic Acid Isolation Kit
The Applied Biosystems™ MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit is developed for scalable, rapid purification of high quality nucleic acid (RNA and DNA) from virus and easy to lyse bacteria in biofluids and transport media samples. You can use the nucleic acid purified with this kit in a broad range of molecular biology downstream applications, such as sequencing and realtime PCR. This protocol guides users through manual isolations in a plate format using a magnetic stand.
RNaseZap™ RNase Decontamination Solution
- Completely removes RNase contamination from glass and plastic surfaces
- Excels at removing high levels of RNase contamination whereas similar products fail
- Proven effective at removing high concentrations of dried-on RNase A
- Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free
Nuclease-Free Centrifuge Tube and Tips - Rigorously tested for RNase-free
Pipette tips and tubes (including PCR tubes, microcentrifuge tubes, and conical tubes) are an easily overlooked source of ribonuclease (RNase) contamination. We offer a range of RNase-free plastic pipette tips, PCR tubes, microcentrifuge tubes, and conical tubes. Each lot of Invitrogen™ Ambion™ brand tips and tubes undergoes rigorous testing and is nuclease-free.
TRIzol™ Reagent
- Permits the isolation of RNA, DNA, and protein from the same sample
- Offers superior lysis capability, even with difficult sample types
- Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
PureLink™ Viral RNA/DNA Mini Kit
- Rapid and efficient purification of high-quality viral nucleic acid using spin column–based centrifugation with no sample cross-contamination
- Specifically designed to purify viral RNA and DNA from ≤500 µL cell-free samples within 45 min
- Ability to elute viral nucleic acids in low elution volumes of 10–50 µL to allow sensitive downstream analysis
Nuclease-Free Water
To meet the special challenges of working with RNA, our Invitrogen waters are certified nuclease-free. They are Type II 18-megohm filtered (via state-of-the-art reverse osmosis filtration and deionization), followed by autoclaving and sterile filtration. The purification equipment used to produce Invitrogen waters is subjected to routine maintenance and testing procedures according to rigid ISO 9001 specifications.
Invitrogen™ SuperScript™ III One-Step RT-PCR Master Mix
- Sensitive—routine detection down to 0.01 pg total RNA (see figure)
- Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
- Specificity—uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers (see figure)
- Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility
Applied Biosystems™ MicroAmp™ Optical 96-Well PCR Plate and Optical Adhesive Film
- Frosted to minimize interfering fluorescence from wells of cycling block
- Screened to eliminate auto-fluorescent plates
- Unique design provides a barrier to ambient air to help insure well-to-well temperature uniformity
- Each reaction plate includes a unique serialized, eight character number label (barcode) that is user-readable and machine-readable to prevent tracking errors
Ion GeneStudio™ S5 High-Throughput Sequencing Platform
The Ion GeneStudio S5 System is a semiconductor-based next-generation sequencing (NGS) system that enables simple targeted sequencing workflows. The Ion GeneStudio S5 System is optimized to provide affordability for labs focused on smaller panels and lower sequencing throughput requirements. With cartridge-based reagents, the system is simple to use and offers scalability and flexibility. It uses Ion 5 Series chips to support a broad range of high-throughput sequencing for clinical research and research applications from microbial genomes and gene panels to exomes and transcriptomes.
Applied Biosystems SeqStudio™ Genetic Analyzer
- Simplified instrument software with interactive touch screen for greater ease of use
- Minimized hands-on setup time with integrated reagents cartridge (SeqStudio Cartridge)
- Application flexibility to run Sanger sequencing and fragment analysis on the same run
- Connectivity: Wi-Fi or wired Ethernet connection
- Web-enabled remote monitoring capability
- Consumables usage tracking through radio frequency identification (RFID)
QuantStudio™ 6/7 Pro Real-Time PCR System
- Easy and convenient hands-free operation with facial authentication and voice commands
- Reduced errors and manual entries with smart qPCR workflow and TaqMan Array Plates with RFID
- Space savings with small footprint and true standalone operation; setup, start, monitor, and analyze directly on the touchscreen
- Peace of mind with Smart Help; contact Technical Support directly from the touchscreen
- Reduced downtime with Smart Remote Support; show issues to Technical Support for speedy resolution
- Application versatility with easily changed blocks and four different block types
- Increased productivity with automation compatibility for 24/7 operation
- Access, analyze, and share data anytime, anywhere with our Connect cloud-based platform for real-time information access, secure data storage, and online results sharing
- Remote monitoring of run status using the Instrument Connect monitoring app
- Design and Analysis software compatible with PC and Mac operating systems
- Compatible with over 8 million TaqMan Assays for gene expression, genetic variation, gene regulation, or protein expression experiments
Thermo Scientific™ NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer
As a new generation of the NanoDrop family, NanoDrop™ One/Onec has carried over NanoDrop’s consistent accuracy and stability. Acclaro technology enhances user understanding of sample quality while delivering accurate quantitative measurements. Early identification of contaminants can prevent failure of downstream applications and save days of troubleshooting.
- Verify sample quality in seconds - identify sample contaminants and obtain corrected concentration results.
- Access information through alerts – receive instant feedback about sample quality with on-demand technical support and guided troubleshooting.
- Rely on accurate results – ensure measurement integrity with embedded sensor and digital image analysis.
- Identify nucleic acid contaminants such as RNA contamination in dsDNA samples and chemical contaminants such as phenol in RNA samples.
Qubit™ Flex Fluorometer and Qubit Reagents
- Improved throughput—measure multiple samples and quantify DNA, RNA, and protein up to 50% faster than single-sample readers
- Flexibility—measure up to eight samples simultaneously
- Complete optimized system—works seamlessly with Qubit reagents to generate reliable, sensitive, and specific results
- Accurate quantification—high levels of accuracy using only 1–20 μL of sample, even with very dilute samples
- On-board calculators—quickly generates Qubit working solution preparation instructions and determines the molarity of your samples
- Easy to use—a large 8-inch, state-of-the-art color touchscreen and intuitive user interface results in easy workflow navigation
- Data export flexibility—data is transferred easily using the included WiFi dongle, a USB drive, or directly to your computer
- Several languages available—choices include English, French, Spanish, Italian, German, simplified Chinese, and Japanese
- Verification test—a pre-loaded program is included to verify performance of the instrument
- Qubit Flex SmartStart Digital Educational Experience—includes interactive visual demonstrations of how to install, operate, and maintain your instrument
- Three-year Extended Warranty—if your instrument needs repair we will send you a factory-certified refurbished replacement
QuantStudio™ 6/7 Pro Real-Time PCR System
- Easy and convenient hands-free operation with facial authentication and voice commands
- Reduced errors and manual entries with smart qPCR workflow and TaqMan Array Plates with RFID
- Space savings with small footprint and true standalone operation; setup, start, monitor, and analyze directly on the touchscreen
- Peace of mind with Smart Help; contact Technical Support directly from the touchscreen
- Reduced downtime with Smart Remote Support; show issues to Technical Support for speedy resolution
- Application versatility with easily changed blocks and four different block types
- Increased productivity with automation compatibility for 24/7 operation
- Access, analyze, and share data anytime, anywhere with our Connect cloud-based platform for real-time information access, secure data storage, and online results sharing
- Remote monitoring of run status using the Instrument Connect monitoring app
- Design and Analysis software compatible with PC and Mac operating systems
- Compatible with over 8 million TaqMan Assays for gene expression, genetic variation, gene regulation, or protein expression experiments
Orbitrap™ Exploris™ 480 Mass Spectrometer
Maximize high-throughput performance in proteomics and biopharma applications with the Thermo Scientific Orbitrap Exploris 480 mass spectrometer. Where next level exploration delivers quantifiable accuracy, and selectivity required to obtain richer insights. Empowering translational scientists by bringing performance and intelligence together, the switch between exploration and targeted quantitation has never been easier. Suddenly your work goes deeper and even the routine will become simple, certain and extraordinary.
mMESSAGE mMACHINE™ T7 ULTRA
The mMESSAGE mMACHINE® T7 Ultra Kit combines a new cap analog, anti-reverse cap analog (ARCA), with a patented high-yield transcription technology to generate RNA transcripts that produce higher protein yields compared to other transcripts upon translation. Advantages of the mMESSAGE mMACHINE® T7 Ultra Kit:
- Synthesize more protein from in vitro-transcribed RNA
- Express RNA transcripts more efficiently both in vitro and in vivo
- Stabilize transcripts in vivo with included reagents for poly(A) tailing
MEGAscript™ T7 Transcription Kit
Intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Km for the enzyme. The MEGAscript® T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.
OEM Solution for AOF Raw Materials
- A complete product line that offers all the components required for in vitro transcription
- All components are animal origin free (AOF)
- Able to provide high-quality customized QC testing
- World-class production capacity with matured quality management system
Small-scale mRNA Purification
Efficient removal of 5S rRNA and tRNA from bacterial RNA with the MEGAclear™ Kit.
MEGAclear™ Transcription Clean-Up Kit
The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:
- Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
- High recovery from 1 ng to 500 µg of input
- Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
- Quick protocol—purify RNA in less than 30 minutes
Purification of mRNA by hybridization to Dynabeads™ Oligo(dT)25. Histogram, showing the yield of mRNA isolated using standard protocol and a 10 times direct up-scaling of both bead amount and buffer volumes.
Dynabeads™ Oligo(dT)25
The oligo(dT)25-coated Dynabeads® specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured. Isolated mRNA is pure, eliminating the need for ribosomal RNA subtraction or a post-extraction DNase treatment. This column-free system ensures the highest transcriptome recovery:
- Physical mRNA capture on mobile magnetic beads
- Rapid and gentle magnetic handling procedures
- No mRNA lost during high g-force spins
- No mRNA trapped in column membranes during elution
Large-scale mRNA Purification
Up-concentration of captured mRNA using DynabeadsTM MyOne Silane. Concentration of mRNA , measured by A260 on the NanoDrop One C instrument, before and after the up concentration step
Dynabeads™ MyOne™ Carboxylic Acid
- Ideal for use a solid support in in vitro diagnostic assays.
- Capture of specific DNA/RNA sequences, proteins or any other analytes.
- High magnetic mobility, low sedimentation rate and higher load: 1.6 mg RNA/mg beads
DynaMag™-50 Magnet
The new DynaMag™-50 magnet is optimized for efficient cell isolation using Dynabeads®. The DynaMag™-50 magnet combines a strong magnetic attraction for efficient separations with a flexible and smart ergonomic design that ensures a comfortable working position. A tilting mechanism on the magnet enables you to rest your elbows while pipetting. The magnet can be used for any starting sample in combination with all types of cell-isolation Dynabeads®.
Performance of the Neon™ system versus conventional electroporation.
Neon™ Transfection System
The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:
- Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
- Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
- Simple—easy to use, with a single reagent kit for all cell types
- Versatile—open system allows electroporation parameters to be optimized freely
Thermo Scientific™ KingFisher™ Flex Automated Nucleic Acid Extraction Instrument
The Thermo Scientific™ KingFisher™ Flex Purification System provides highly versatile and reproducible purification of 24 or 96 samples per run. It can be used with a variety of reagents, including Dynabeads products or MagMAX nucleic acid extraction kits, enabling scientists to process samples for a variety of applications.
Applied Biosystems™ MagMAX™ Virus/Pathogen Nucleic Acid Isolation Kit
The Applied Biosystems™ MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit is developed for scalable, rapid purification of high quality nucleic acid (RNA and DNA) from virus and easy to lyse bacteria in biofluids and transport media samples. You can use the nucleic acid purified with this kit in a broad range of molecular biology downstream applications, such as sequencing and realtime PCR. This protocol guides users through manual isolations in a plate format using a magnetic stand.
RNaseZap™ RNase Decontamination Solution
- Completely removes RNase contamination from glass and plastic surfaces
- Excels at removing high levels of RNase contamination whereas similar products fail
- Proven effective at removing high concentrations of dried-on RNase A
- Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free
Nuclease-Free Centrifuge Tube and Tips - Rigorously tested for RNase-free
Pipette tips and tubes (including PCR tubes, microcentrifuge tubes, and conical tubes) are an easily overlooked source of ribonuclease (RNase) contamination. We offer a range of RNase-free plastic pipette tips, PCR tubes, microcentrifuge tubes, and conical tubes. Each lot of Invitrogen™ Ambion™ brand tips and tubes undergoes rigorous testing and is nuclease-free.
TRIzol™ Reagent
- Permits the isolation of RNA, DNA, and protein from the same sample
- Offers superior lysis capability, even with difficult sample types
- Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
PureLink™ Viral RNA/DNA Mini Kit
- Rapid and efficient purification of high-quality viral nucleic acid using spin column–based centrifugation with no sample cross-contamination
- Specifically designed to purify viral RNA and DNA from ≤500 µL cell-free samples within 45 min
- Ability to elute viral nucleic acids in low elution volumes of 10–50 µL to allow sensitive downstream analysis
Nuclease-Free Water
To meet the special challenges of working with RNA, our Invitrogen waters are certified nuclease-free. They are Type II 18-megohm filtered (via state-of-the-art reverse osmosis filtration and deionization), followed by autoclaving and sterile filtration. The purification equipment used to produce Invitrogen waters is subjected to routine maintenance and testing procedures according to rigid ISO 9001 specifications.
Invitrogen™ SuperScript™ III One-Step RT-PCR Master Mix
- Sensitive—routine detection down to 0.01 pg total RNA (see figure)
- Convenience—one-step format for speed, convenience, and less reaction-to-reaction variability
- Specificity—uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers (see figure)
- Amplicon size—compatible detection of targets up to 4.5 kb in length for greater flexibility
Applied Biosystems™ MicroAmp™ Optical 96-Well PCR Plate and Optical Adhesive Film
- Frosted to minimize interfering fluorescence from wells of cycling block
- Screened to eliminate auto-fluorescent plates
- Unique design provides a barrier to ambient air to help insure well-to-well temperature uniformity
- Each reaction plate includes a unique serialized, eight character number label (barcode) that is user-readable and machine-readable to prevent tracking errors
Ion GeneStudio™ S5 High-Throughput Sequencing Platform
The Ion GeneStudio S5 System is a semiconductor-based next-generation sequencing (NGS) system that enables simple targeted sequencing workflows. The Ion GeneStudio S5 System is optimized to provide affordability for labs focused on smaller panels and lower sequencing throughput requirements. With cartridge-based reagents, the system is simple to use and offers scalability and flexibility. It uses Ion 5 Series chips to support a broad range of high-throughput sequencing for clinical research and research applications from microbial genomes and gene panels to exomes and transcriptomes.
Applied Biosystems SeqStudio™ Genetic Analyzer
- Simplified instrument software with interactive touch screen for greater ease of use
- Minimized hands-on setup time with integrated reagents cartridge (SeqStudio Cartridge)
- Application flexibility to run Sanger sequencing and fragment analysis on the same run
- Connectivity: Wi-Fi or wired Ethernet connection
- Web-enabled remote monitoring capability
- Consumables usage tracking through radio frequency identification (RFID)
QuantStudio™ 6/7 Pro Real-Time PCR System
- Easy and convenient hands-free operation with facial authentication and voice commands
- Reduced errors and manual entries with smart qPCR workflow and TaqMan Array Plates with RFID
- Space savings with small footprint and true standalone operation; setup, start, monitor, and analyze directly on the touchscreen
- Peace of mind with Smart Help; contact Technical Support directly from the touchscreen
- Reduced downtime with Smart Remote Support; show issues to Technical Support for speedy resolution
- Application versatility with easily changed blocks and four different block types
- Increased productivity with automation compatibility for 24/7 operation
- Access, analyze, and share data anytime, anywhere with our Connect cloud-based platform for real-time information access, secure data storage, and online results sharing
- Remote monitoring of run status using the Instrument Connect monitoring app
- Design and Analysis software compatible with PC and Mac operating systems
- Compatible with over 8 million TaqMan Assays for gene expression, genetic variation, gene regulation, or protein expression experiments
Thermo Scientific™ NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer
As a new generation of the NanoDrop family, NanoDrop™ One/Onec has carried over NanoDrop’s consistent accuracy and stability. Acclaro technology enhances user understanding of sample quality while delivering accurate quantitative measurements. Early identification of contaminants can prevent failure of downstream applications and save days of troubleshooting.
- Verify sample quality in seconds - identify sample contaminants and obtain corrected concentration results.
- Access information through alerts – receive instant feedback about sample quality with on-demand technical support and guided troubleshooting.
- Rely on accurate results – ensure measurement integrity with embedded sensor and digital image analysis.
- Identify nucleic acid contaminants such as RNA contamination in dsDNA samples and chemical contaminants such as phenol in RNA samples.
Qubit™ Flex Fluorometer and Qubit Reagents
- Improved throughput—measure multiple samples and quantify DNA, RNA, and protein up to 50% faster than single-sample readers
- Flexibility—measure up to eight samples simultaneously
- Complete optimized system—works seamlessly with Qubit reagents to generate reliable, sensitive, and specific results
- Accurate quantification—high levels of accuracy using only 1–20 μL of sample, even with very dilute samples
- On-board calculators—quickly generates Qubit working solution preparation instructions and determines the molarity of your samples
- Easy to use—a large 8-inch, state-of-the-art color touchscreen and intuitive user interface results in easy workflow navigation
- Data export flexibility—data is transferred easily using the included WiFi dongle, a USB drive, or directly to your computer
- Several languages available—choices include English, French, Spanish, Italian, German, simplified Chinese, and Japanese
- Verification test—a pre-loaded program is included to verify performance of the instrument
- Qubit Flex SmartStart Digital Educational Experience—includes interactive visual demonstrations of how to install, operate, and maintain your instrument
- Three-year Extended Warranty—if your instrument needs repair we will send you a factory-certified refurbished replacement
QuantStudio™ 6/7 Pro Real-Time PCR System
- Easy and convenient hands-free operation with facial authentication and voice commands
- Reduced errors and manual entries with smart qPCR workflow and TaqMan Array Plates with RFID
- Space savings with small footprint and true standalone operation; setup, start, monitor, and analyze directly on the touchscreen
- Peace of mind with Smart Help; contact Technical Support directly from the touchscreen
- Reduced downtime with Smart Remote Support; show issues to Technical Support for speedy resolution
- Application versatility with easily changed blocks and four different block types
- Increased productivity with automation compatibility for 24/7 operation
- Access, analyze, and share data anytime, anywhere with our Connect cloud-based platform for real-time information access, secure data storage, and online results sharing
- Remote monitoring of run status using the Instrument Connect monitoring app
- Design and Analysis software compatible with PC and Mac operating systems
- Compatible with over 8 million TaqMan Assays for gene expression, genetic variation, gene regulation, or protein expression experiments
Orbitrap™ Exploris™ 480 Mass Spectrometer
Maximize high-throughput performance in proteomics and biopharma applications with the Thermo Scientific Orbitrap Exploris 480 mass spectrometer. Where next level exploration delivers quantifiable accuracy, and selectivity required to obtain richer insights. Empowering translational scientists by bringing performance and intelligence together, the switch between exploration and targeted quantitation has never been easier. Suddenly your work goes deeper and even the routine will become simple, certain and extraordinary.
mMESSAGE mMACHINE™ T7 ULTRA
The mMESSAGE mMACHINE® T7 Ultra Kit combines a new cap analog, anti-reverse cap analog (ARCA), with a patented high-yield transcription technology to generate RNA transcripts that produce higher protein yields compared to other transcripts upon translation. Advantages of the mMESSAGE mMACHINE® T7 Ultra Kit:
- Synthesize more protein from in vitro-transcribed RNA
- Express RNA transcripts more efficiently both in vitro and in vivo
- Stabilize transcripts in vivo with included reagents for poly(A) tailing
MEGAscript™ T7 Transcription Kit
Intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Km for the enzyme. The MEGAscript® T7 Kit typically yields over 10 times more RNA than conventional in vitro transcription reactions. The kit is not recommended for synthesis of high specific activity probes.
OEM Solution for AOF Raw Materials
- A complete product line that offers all the components required for in vitro transcription
- All components are animal origin free (AOF)
- Able to provide high-quality customized QC testing
- World-class production capacity with matured quality management system
Small-scale mRNA Purification
Efficient removal of 5S rRNA and tRNA from bacterial RNA with the MEGAclear™ Kit.
MEGAclear™ Transcription Clean-Up Kit
The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:
- Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
- High recovery from 1 ng to 500 µg of input
- Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
- Quick protocol—purify RNA in less than 30 minutes
Purification of mRNA by hybridization to Dynabeads™ Oligo(dT)25. Histogram, showing the yield of mRNA isolated using standard protocol and a 10 times direct up-scaling of both bead amount and buffer volumes.
Dynabeads™ Oligo(dT)25
The oligo(dT)25-coated Dynabeads® specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured. Isolated mRNA is pure, eliminating the need for ribosomal RNA subtraction or a post-extraction DNase treatment. This column-free system ensures the highest transcriptome recovery:
- Physical mRNA capture on mobile magnetic beads
- Rapid and gentle magnetic handling procedures
- No mRNA lost during high g-force spins
- No mRNA trapped in column membranes during elution
Large-scale mRNA Purification
Up-concentration of captured mRNA using DynabeadsTM MyOne Silane. Concentration of mRNA , measured by A260 on the NanoDrop One C instrument, before and after the up concentration step
Dynabeads™ MyOne™ Carboxylic Acid
- Ideal for use a solid support in in vitro diagnostic assays.
- Capture of specific DNA/RNA sequences, proteins or any other analytes.
- High magnetic mobility, low sedimentation rate and higher load: 1.6 mg RNA/mg beads
DynaMag™-50 Magnet
The new DynaMag™-50 magnet is optimized for efficient cell isolation using Dynabeads®. The DynaMag™-50 magnet combines a strong magnetic attraction for efficient separations with a flexible and smart ergonomic design that ensures a comfortable working position. A tilting mechanism on the magnet enables you to rest your elbows while pipetting. The magnet can be used for any starting sample in combination with all types of cell-isolation Dynabeads®.
Performance of the Neon™ system versus conventional electroporation.
Neon™ Transfection System
The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:
- Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
- Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
- Simple—easy to use, with a single reagent kit for all cell types
- Versatile—open system allows electroporation parameters to be optimized freely
Featured Webinars
On-demand: Accurate, Rapid Typing of the SARS-CoV-2 Genome with Ion AmpliSeq Technology
In this collection of webinars, researchers demonstrate how NGS can be used to interrogate the SARS-CoV-2 genome across multiple samples simultaneously for contact tracing and to rapidly obtain a global picture for surveillance, as well as other applications such as phylogenetic analysis, viral typing, and environmental monitoring.
On-demand: Critical Raw Material for mRNA-based Vaccine Development
Accelerated development of mRNA-based vaccine has necessitated the transformation of standard, research grade in vitro transcription reagents into raw material specifically designed and manufactured under advanced quality standards. The TheraPure grade reagents for mRNA-based vaccine represents Thermo Fisher Scientific’s highest level of purity for mRNA synthesis reagents, with products manufactured using high-definition analytics and tightly controlled purity standards. The TheraPure grade portfolio offers a unique integrated solution for mRNA synthesis, including animal origin-free and ampicillin-free enzymes.
