With the Thermo Scientific Phusion Plus DNA Polymerase, expect to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance.
In addition, its specialized reaction buffer allows PCR amplification without the need to calculate primer annealing temperatures, helping you save time and avoid mistakes in PCR runs.
Features:
Convenient setup—allows for a universal annealing temperature of 60°C
High fidelity—provides >100x higher sequence accuracy than Taq polymerase
Enhanced specificity—reduces nonspecific amplification and primer degradation via hot-start modification
Benchtop stability—enables lab automation setup since assembled reactions are stable at room temperature for 24 hours
High inhibitor tolerance—works with DNA of suboptimal purity
Invitrogen™ TOPO™ PCR Cloning Kits are trusted to provide a fast, simple, and highly efficient way to clone PCR-amplified products without the need for any additional ligation steps.
No matter what your downstream application is, TOPO PCR cloning solutions help you get the right clone sooner, freeing up your time to answer more important questions.
Features:
Efficient—up to 95% of clones contain desired insert
Fast—5 min reaction at room temperature
Easy—add restriction sites and/or universal primer sites to either end of your PCR product in just 3 easy steps
Flexible—available in a variety of formats and sizes for multiple applications
Invitrogen has the largest variety of competent cells. Choosing the ideal competent cells for your cloning applications and workflows is a critical component of success. Find the right strain for your research. Each E. coli strain is specially engineered to keep you moving ahead.
Options available:
One Shot – Transformation and recovery in the same tube.
MultiShot StripWell – 12 strips of 8 tubes in a 96 well format; do as many or as few reactions as needed
MultiShot FlexPlate – 96 well plate divisible into 12 x 8-well segments. Manual or automated applications
MultShoti – 96-well microarray plates fit automated format
This promotion is open only to customers in Singapore.
Customers who purchase 2 of the qualifying products listed above will be entitled to a 10% discount off list price and a free Fuel Your Research Play. Products purchased must be from different categories. In order to redeem the discount and free play, the promotion code RMOL22 must be presented at time of order no later than 30 September 2022, or before promotional supplies are depleted, whichever comes first.
Customers who purchase 3 of the qualifying products listed above will be entitled to a 20% discount off list price and a free Fuel Your Research Play. Products purchased must be from different categories. In order to redeem the discount and free play, the promotion code RCLO22 must be presented at time of order no later than 30 September 2022, or before promotional supplies are depleted, whichever comes first.
Customers who purchase 4 of the qualifying products listed above will be entitled to a 30% discount off list price and a free Fuel Your Research Play. Products purchased must be from different categories. In order to redeem the discount and free play, the promotion code RPRO22 must be presented at time of order no later than 30 September 2022, or before promotional supplies are depleted, whichever comes first.
The QR code for the free play will be sent to the email address from which the order is sent from. Due to the redemption process, please allow 2 weeks for the QR code to be sent. There is no limit to the number of redemptions a lab may make. To find out where our Fuel Your Research Kiosks are, please visit thermofisher.com/FuelYourResearch. Cannot be combined with other discounts or promotions. Limited to Research Use Only products. By participating in the promotion, you warrant that you are not prohibited by employment, contract, or law from accepting a gift from Thermo Fisher Scientific. Healthcare professionals may not participate in this promotion . Offer void where prohibited, licensed or restricted by local laws or regulation or agency/institutional policy. Other restrictions may apply. Thermo Fisher Scientific reserves the right to change these terms and conditions at any time without prior notice.