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U-2 OS cells were transduced with Premo™ Autophagy sensor LC3B-RFP (red) and CellLight® Tubulin-GFP (green) and cultured overnight. The following day cells were treated with 60uM Chloroquine and cultured overnight. Cells were then counterstained with 1ug/mL Hoeschst 33342 (blue) and imaged on a Delta Vision wide field microscope.
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
Endothelial cells. Go ›
1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
BPAE cells fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit. Go ›
Live cell imaging with CellLight™ reagents. Go ›
Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›
CD335 (NKp46) Antibody (63335182) in RE Go ›
CD223 (LAG-3) Antibody (56223942) in TM Go ›
Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›
Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›
Using anti-LC3B and the Click-iT® technology autophagy detected and glycoproteins labeled in HeLa cells Go ›