Hydrophobic Interaction Chromatography

Thermo Scientific POROS hydrophobic interaction chromatography (HIC) resins are based on the 50 μm POROS base bead, utilizing a novel coating procedure to enable functionalization with unique hydrophobic ligands. These resins are suitable for bind/elute and flow-through applications at lower salt concentrations and have higher binding capacity and resolution than classical HIC resins, thereby enabling more flexibility around process operating conditions.

Key features 

• Three unique resins covering a wide range of hydrophobicity
• High resolution, even with lower conductivity conditions
• High dynamic binding capacity and superior pressure-flow characteristics

Key applications

• Monoclonal antibodies, bispecific antibodies, and antibody–drug conjugates (ADCs) 
• Product-related impurities and aggregate removal
• Plasmids, RNAi, and oligonucleotides

Thermo Fisher Scientific provides a suite of POROS HIC resins offering a differentiating range in hydrophobicity suitable for bind/elute and flow-through applications. The resins can be used for the purification of a wide variety of biomolecules including therapeutic proteins, antibody fragments, antibody drug conjugates (ADCs), and other large molecules.

The 50 µm resin backbone consists of cross‐linked poly(styrene-divinylbenzene) with a unique pore structure that provides rapid mass transport and enables enhanced productivity. The particle surface is coated with a novel polymer coating, which is then further derivatized with a range of hydrophobic ligands for flexible purification process design. POROS HIC resins enable outstanding performance independent of flow rate, a major improvement in the purification tool box for impurity removal.

• Differentiating selectivity and superior resolution capability which helps improve yield and purity and may help reduce cost of goods
• High capacity for a range of molecules leading to reduced column size and smaller footprint
• Use of lower salt concentrations and weaker lyotropic salts allowing for increased process optimization flexibility with lower conductivity while maintaining exceptional resolution
• Flow rate independent performance
• Robust stability

Thermo Fisher Scientific provides a suite of POROS HIC resins offering a differentiating range in hydrophobicity suitable for bind/elute and flow-through applications. The resins can be utilized at lower salt concentrations for the purification of a wide variety of biomolecules including therapeutic proteins, antibody fragments, antibody drug conjugates (ADCs), and other large molecules.