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Fluorescence Microscopy ReagentsProven imaging fluorescent reagents for the detection of cellular components, organelles, and nucleic acids Build imaging experiment |
Imaging applications
Fixed cell | Organoid 3D cells | Live-cell | IHC
Immunofluorescence | Super resolution | Spatial biology
Imaging reagents detecting cell structures
Using fluorescent dyes, probes, and reagents allows researchers to gain insights into various aspects of cellular biology, such as cellular function, organelle structure, and detect proteins, nucleic acids, lipids, and carbohydrates. These fluorescent reagents can be combined with other dyes or antibodies, enabling multiplex analysis. This multiplexing is achieved by selecting appropriate fluorophores that can be excited at distinct wavelengths and using microscopes equipped with suitable filters to separate the emitted fluorescence signals. By leveraging these reagents, researchers can obtain complex and comprehensive information about cellular processes and structures.
Explore fluorescence microscopy reagents and dyes identifying cellular function and organelle structures:
Cell painting assay reagents
Cell painting assays are available that combine multiple cellular reagents for high-content screening.
Imaging reagents for apoptosis analysis
Various approaches can used to study apoptosis due to the complex and dynamic nature of programmed cell death cascades. There are an array of fluorescent assays and reagents that can be used to detect these apoptotic process. These assays and reagents can be combined with other dyes or antibodies, each excited and emitted at distinct wavelengths. The fluorescent signals can be visualized using microscopes equipped with suitable filters. This multi-parametric approach allows for a comprehensive understanding of the mechanism involved in cell death.
Explore fluorescence microscopy assays and reagents identifying cells undergoing apoptosis:
Interested in the non-apoptotic form of cell death? Learn more about Ferroptosis Research Solutions
Fluorophore technology for microscopy experiments
Learn about fluorophore selection and understand how to pick the correct fluorophore technology for your imaging experiments.
Overview of Alexa Fluor Dyes
Choose Alexa Fluor fluorescent dyes for your imaging experiments by viewing a selection guide and examining spectral information.
Overview of Tyramide Amplification Kits and Reagents
Having issues detecting your low abundant proteins? Understand how to amplify the signal of fluorescent proteins using the tyramide amplification method.
Click-iT EdU Assays for cellular proliferation
Discover a faster and gentler BrdU alternative to measure cell proliferation with Click-iT EdU assays. Read the selection guide and read the protocol.
ViewRNA Assays for RNA detection
Looking to image your RNA transcripts? Use our commercially available in-situ hybridization assay to visualize RNA in formalin-fixed paraffin-embedded tissue.
Curing Antifade Mountants
Want to prevent fluorescence bleaching? Looking for a more permanent mountant? Read about Prolong hard set antifade mountants.
Non-Setting Antifade Mountants
Want to wash your mountant and re-probe your sample? Understand the properties of SlowFade antifade mountants.
Microscopy protocols
Using fluorescent reagents is often less complex compared to other techniques and are more easily detected. Typical labeling times span between 5 and 60 mins. Certain fluorophore signal can be retained with fixation, and a number can be used for live-cell imaging. The protocols and reagents described in imaging protocol handbook and linked below are to be used as a guide only, and may require optimization for your experiment.
Popular microscopy protocols include:
- Immunofluorescent staining of intracellular antigens on cultured cells
- BrdU labeling and detection protocol
- Click-iT Plus EdU Imaging Kits protocol
- Image-iT DEAD Green Kit protocol
- LIVE/DEAD Cell Imaging Kit (488/570) protocol
- ReadyProbes Cell Viability Imaging Kit, Blue/Green protocol
- ActinGreen 488 ReadyProbes Reagent protocol
- DAPI protocol for fluorescence imaging
- NucGreen Dead 488 ReadyProbes Reagent protocol for fixed cells
Imaging tools
Find helpful tools below to help you plan your imaging experiments.
SpectraViewer Supports all levels of experimental complexity, use the tool to compare excitation and emission spectra of fluorophores and reagents.
See fluorophores
Stain-iT Cell Staining Simulator Visualize staining your cell without wasting your reagents, antibodies, or time.
Resources
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For Research Use Only. Not for use in diagnostic procedures.