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Cell Viability Information
Dead cells often give false positive results, because many reagents will bind to them non-specifically. Therefore, removing dead cells from your flow cytometry data is a critical step to ensure accurate results and analysis. In this section you will find educational resources for analyzing the viability of cells using flow cytometry including application notes, articles, scientific posters, videos, webinars and more.
Cell viability features
On demand webinar
An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
BioProbes article
A comprehensive resource for state-of-the-art flow cytometry methods
This article provides a review of recently published and extensive guidelines for the use of flow cytometry and cell sorting in immunological studies, as well as an infographic giving tips for key data controls.
Cell viability learning resources
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| Type | Title | Categories |
|---|---|---|
| BioProbes articles (Issues 50–present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Molecular Probes Handbook | Viability and cytotoxicity assay reagents—Section 15.2 | cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader |
| Molecular Probes Handbook | Viability and cytotoxicity assay kits for diverse cell types—Section 15.3 | cell health, cell viability and function, fluorescence microscopy/fluorescence imaging, microplate reader |
| Protocols | LIVE/DEAD Fixable Dead Cell Stains protocol | flow cytometry, viability |
| Protocols | LIVE/DEAD Sperm Viability Kit flow cytometry protocol | flow cytometry, viability |
| Protocols | LIVE/DEAD Violet Viability/Vitality Kit protocol | flow cytometry, viability |
| Protocols | SYTOX Dead Cell Stains protocol | cell viability, flow cytometry |
| Protocols | Viability staining protocol for flow cytometry (Invitrogen eBioscience reagents) | cell viability, flow cytometry |
| Scientific poster (2018) | Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia | antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research |
| Scientific poster (2018) | Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques | Attune/Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination |
| Scientific Poster (2016) | Cell mediated cytotoxicity in “untouched” whole blood | Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood |
| Scientific poster (2013) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | apoptosis, ArrayScan, caspase substrates, cell health, cells, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2009) | A simple, fast and quantitative single-step dead-cell indicator for flow cytometry | apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability |
| Scientific poster (2009) | Dead cell stains in flow cytometry: A comprehensive analysis | apoptosis, cells, flow cytometry, fluorescent dyes, immunophenotyping, viability |
| Scientific poster (2006) | Novel violet-excited reagents for detection of viability and vitality | Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents |
| CYToMORE lecture video (2016) | Novel reagent solutions for flow cytometry Presented by Jolene Bradford, Thermo Fisher Scientific, USA | Attune NxT, flow cytometry assays |
| Webinar | An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes. | apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability |