green, blue, red, yellow and pink color laser lights intersecting at multiple points

Advances in both flow cytometry reagents and instrumentation allow researchers to run increasingly complex multicolor experiments. One of the biggest challenges in multiparameter flow cytometry is selecting the right combination of fluorophores and antibody conjugates so that the need for compensation and spillover adjustments is kept to a minimum while the quality and accuracy of the data are not compromised. In this section you will find educational resources and tools to help you design and execute multicolor flow cytometry experiments, including panel design tool, articles, application notes, on demand webinars, and more.

Panel design and multicolor flow cytometry features


Panel design and multicolor flow cytometry learning resources

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TypeTitleCategories
Application note (2015)Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensationAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)Detection of murine regulatory T cells on the Attune NxT Flow CytometerAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow CytometerAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015)Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cellsAttune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry
Application note (2015) 13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
BioProbes articles (Issues 50– present day)BioProbes Journal of Cell Biology Applicationcell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
Scientific Poster (2017) T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright
Scientific poster (2009) A comparative study of 25 different CD4 conjugates Alexa Fluor, antibodies, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
CYToMORE lecture video (2016)Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy 
Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy
flow cytometry, immunology, immunophenotyping
Tutorial videoMolecular Probes Tutorial Series—Anatomy of Fluorescence Spectra absorbance, emission, excitation, spectra
Tutorial videoMolecular Probes Tutorial Series—Introduction to Flow Cytometry flow cytometer calibration, multicolor flow cytometry
Tutorial videoMolecular Probes Tutorial Series—Introduction to Fluorescence flow cytometry, fluorescence imaging
Tutorial videoMolecular Probes Tutorial Series—Overview of Filters and Light Sources emission, excitation, filters, light cubes, light sources
WebinarBasics of flow cytometry, Part I: Gating and data analysis 
An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry.
flow cytometry data, flow cytometry set up, multicolor flow cytometry
WebinarBasics of multi color flow cytometry panel design 
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including:
  • Rules for designing panels
  • Examples and practical application of these rules
  • Controls and standardization
  • Relevance of panel design to new mass cytometry platforms
antibodies, compensation, multicolor flow cytometry

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.