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Phosphoramidites for Oligonucleotide Synthesis |
Thermo Scientific phosphoramidites are built to help meet varying needs of oligonucleotide synthesis from research applications to commercial manufacturing for diagnostic and therapeutic developers. For research and early development, there are over 136 ready-to-ship phosphoramidites (abbreviated as amidites below) of small pack sizes in the online catalog.
Custom solutions and modification capabilities are available to support you from the beginning at the discovery phase. Our core scale-up competencies help ensure continuity all the way to the commercial volume needed for go-to-market. With manufacturing expertise in custom nucleic acid chemistry for more than 40 years, our technical experts help ensure the consistent quality and security of supply chain for scaling up from grams to metric tons.
Comprehensive supply of phosphoramidites
Amidites are the building blocks used in chemical synthesis of oligonucleotides. Optimize your oligo synthesis and manufacturing process development with our large selection of amidites offerings that are also available in two quality grades.
2’ Modified RNA phosphoramidites
Common base modifications—2′-MOE, 2′-OMe, and 2′-fluoro—for the synthesis of antisense oligos and siRNAs.
DNA phosphoramidites
For synthesis of DNA oligos for diagnostic assays and therapeutic applications.
RNA phosphoramidites
Synthesis of RNA oligos for diagnostic assays and therapeutic applications.
Dye-labeled phosphoramidites
With fluorescent labels for synthesis of oligos for detection in molecular assays and microarrays.
Linkers and spacers
Linkers at 3′, 5′, or internal positions for NHS ester conjugation; and spacers to increase conformational support.
Structural phosphoramidites
With modifications (e.g., 5m-dC, N6-Me-dA, N4-Ethyl-dC, 8-oxo-dG) for synthesis of primers, probes, or oligos.
Succinates
DNA succinates for the derivatization of controlled pore glass, polystyrene, or other supports used in solid-phase oligo synthesis.
Oligonucleotide therapeutics
Custom services and product offerings to enable development from start to final commercialization.
Quality attributes of TheraPure chemistry solutions
Processes are monitored from starting material procurement to final QC and product release. Our rigorously controlled manufacturing processes help maximize batch-to-batch reproducibility and minimize levels of reactive P(III) impurities, which could accumulate as by-products into the oligonucleotide during synthesis.
| TheraPure | Standard | |
|---|---|---|
| Recommended application |
|
|
| Animal origin free (AOF) manufacturing | Manufactured with animal origin free (AOF) materials | |
| HPLC purity | 99% or higher with unknown, non-critical, and critical impurity specifications | 98% |
| 31P NMR purity | 99% or higher with unknown, non-critical, and critical impurity specifications | 98% |
| Water content | 0.3% or lower | |
| Solution clarity | 0.2 M solution in acetonitrile is free of undissolved particulates | – |
| UV spectral identification | The λ maxima of the sample and standard spectra are within ±2 nm | – |
| Residual solvents | 4.0% or lower | – |
Analytical data example: TheraPure Bz-dA phosphoramidite
Phosphoramidites are the key raw material and building blocks of oligonucleotide therapeutics. Incorporating the quality testing step into the manufacturing process development from the beginning is a best practice for developing therapeutics or diagnostics from discovery to commercialization. The comprehensive QC processes include the analysis of phosphoramidites that is crucial to monitoring the impurity levels.
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Typical 31P NMR scan of a TheraPure RNA phosphoramidite
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Impurity profile example of TheraPure 2ʹ-TBDMS-iBU-rG phosphoramidite by LC-MS
Manufacturing capabilities
Technical expertise
Over 40 years of expertise in custom chemistry and scale-up experience for innovative projects.
Security of supply
5x capacity expansion to enable emerging diagnostic assays and oligo therapeutics.
Refined quality
ISO 9001:2015–certified quality management system for nucleic acid products
Quality manufacturing and documentation
Batch-to-batch consistency, minimal level of reactive impurities, and analytical documentation support.
Service excellence
48 hours or less response to orders, >98% delivery to customer at promised date*.
Flexible packaging
Multiple customization formats of synthesizers, solvent methods, capping, fill volumes, and more.
*May not be available in all regions
Hear from the experts
Our scientists discuss the history of Thermo Fisher's Milwaukee facility, customer partnership, manufacturing process, quality improvement, mitigation of supply chain issues, and the recent response to global needs.
Quick tour of the Thermo Fisher Scientific facility at Milwaukee for phosphoramidites and nucleotides
Elevating product values with expertise, partnership, and commitment
Applications and manufacturing process of phosphoramidites and nucleotides
Improving and delivering quality in phosphoramidites
Custom services for oligonucleotide chemistry
In addition to our Thermo Scientific phosphoramidites, we offer custom services for oligonucleotide chemistry tailored to fit your needs in diagnostic and therapeutic applications. We are fully set up to provide customization of our existing phosphoramidites, synthesis of new compounds, and development of oligonucleotide delivery chemistries. Simply fill out this short form with your interest so that our representative can explore available options with you.
Advanced project management
- Customer involvement
- Dedicated cross-functional project team
- Project planning and execution tailored to each custom request
- Manufactured in an ISO 9001–registered facility
- Thoroughly documented processes provide consistent product quality with batch-to-batch consistency
Customization options
- Base or base protection modification
- Sugar modifications
- Changes to phosphitylation, or phosphitylation of custom molecules
- 5′ protection modification
Custom chemistries and capabilities
- Custom phosphoramidites, delivery chemistries, modified nucleotides, and reagents
- Modifiers and linkers
- R&D to large-scale manufacturing
- Development of customized QC
- Custom filling, labeling, and packaging
 | Phosphoramidite customization options A. Dimethoxytrityl (DMT) substitution with alternate protecting groups B. Base = A, C, G, T, or U protection modifications or base modifications C. Sugar modifications and protection options |
TheraPure services—access to customized materials for producing nucleic acid therapeutics
Options for base protection groups and package formats
Depending on your selected phosphoramidites, they may be available in one or more of these options. Please contact us if you do not find one that meets your needs.
Base N-protections to choose depending on your deprotection methods, their sensitivity to base hydrolysis, and their reactivity with tags and labels:
| Package formats to accommodate your synthesis scales, synthesizers, and solvation methods:
| |
Resources
Technical Spotlights
Custom Services and Quotation
Speak with a technical specialist about support available for the oligonucleotide manufacturing process development of your unique projects.
Phosphoramidite Learning Resource
Access webinars, videos, articles, and more to optimize your success in oligonucleotide synthesis for diagnostic and therapeutic manufacturing process development.
mRNA Production Learning Library
Browse the educational assets related to raw materials selection, process development using in vitro transcription, scale-up synthesis, and the latest trends in mRNA production.
For Research Use Only. Not for use in diagnostic procedures.
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