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TaqMan Array MicroRNA Cards are the ideal solution for profiling large numbers of miRNAs. Benefits include:
Our TaqMan Advanced miRNA chemistry on a TaqMan Array Card or OpenArray Plate. Enable highly sensitive and specific quantification of miRNAs in a high throughput format.
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Taqman™ OpenArray Human Advanced MicroRNA Panel ›
Taqman array microRNA cards using classic chemistry include coverage of the most relevant microRNA for human or rodent species, aligned with Sanger miRBase v22, with over 740 assays enabled across the sets of TaqMan™ Array MicroRNA Cards (Cards A and B).
This product requires the use of Megaplex™ Primer Pools for miRNA reverse transcription. Optional Megaplex™ PreAmp Primers can be used for preamplification of limited samples prior to real-time PCR using the TaqMan Array MicroRNA Card.
TaqMan Array miRNA Cards using the Advanced chemistry enable accurate quantitation of the most common human mature miRNAs, mapped to miRBase v22. The Human A and B Cards contain 754 unique miRNAs. The Human Endogenous Controls Card contains 32 unique microRNA control targets. The Human Serum/Plasma Card contains 192 unique microRNA targets known to be present in serum/plasma.
Custom miRNA array cards are available in both Classic and Advanced chemistries. They can hold from 12 to 380 assays, and 1 to 8 samples on each array card. Ten formats are offered, available only with microfluidics card design.
Applied Biosystems offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).
Recommended Products
TaqMan miRNA ABC Purification ™Kit | ||
mirVana™ miRNA Isolation Kit | ||
RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE | ||
TaqMan™ MicroRNA Cells-to-CT™ Kit |
Designed to streamline miRNA profiling, Megaplex™ RT Primers are ideal for global miRNA expression profiling; just two pools provide comprehensive coverage of the Sanger miRBase database content for human and rodent. The content of each pool matches corresponding TaqMan® Array Human or Rodent MicroRNA Cards (Step 4). The TaqMan® MicroRNA Reverse Transcription (RT) Kit provides optimal TaqMan® MicroRNA Assay performance. This kit is used with the single RT primer provided with an individual TaqMan® MicroRNA Assay, or with Megaplex™ RT Primers, to convert miRNA to cDNA prior to real-time PCR quantitation.
Megaplex™ PreAmp Primers generate a comprehensive miRNA expression profile using as little as 1 ng of input total RNA. With content matched to specific Megaplex™ RT Primers and TaqMan® Array Human or Rodent MicroRNA Cards, cDNA prepared by reverse transcription can be preamplified using Megaplex™ PreAmp Primers and TaqMan® PreAmp Master Mix to uniformly amplify all miRNAs present in the original sample significantly increasing assay sensitivity.
Megaplex™ PreAmp Primers | ||
TaqMan® PreAmp Master Mix |
For the final quantitation step, TaqMan® Universal Master Mix II is added to each sample and pipetted into the sample loading ports of a TaqMan® Array Card, greatly simplifying assay set-up and minimizing variability due to pipetting.
TaqMan® Array MicroRNA Cards
|
TaqMan® Universal Master Mix II
|
Recommended Products
Applied Biosystems offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).
Recommended Products
TaqMan miRNA ABC Purification ™Kit | ||
mirVana™ miRNA Isolation Kit | ||
RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE | ||
TaqMan™ MicroRNA Cells-to-CT™ Kit |
Designed to streamline miRNA profiling, Megaplex™ RT Primers are ideal for global miRNA expression profiling; just two pools provide comprehensive coverage of the Sanger miRBase database content for human and rodent. The content of each pool matches corresponding TaqMan® Array Human or Rodent MicroRNA Cards (Step 4). The TaqMan® MicroRNA Reverse Transcription (RT) Kit provides optimal TaqMan® MicroRNA Assay performance. This kit is used with the single RT primer provided with an individual TaqMan® MicroRNA Assay, or with Megaplex™ RT Primers, to convert miRNA to cDNA prior to real-time PCR quantitation.
Megaplex™ PreAmp Primers generate a comprehensive miRNA expression profile using as little as 1 ng of input total RNA. With content matched to specific Megaplex™ RT Primers and TaqMan® Array Human or Rodent MicroRNA Cards, cDNA prepared by reverse transcription can be preamplified using Megaplex™ PreAmp Primers and TaqMan® PreAmp Master Mix to uniformly amplify all miRNAs present in the original sample significantly increasing assay sensitivity.
Megaplex™ PreAmp Primers | ||
TaqMan® PreAmp Master Mix |
For the final quantitation step, TaqMan® Universal Master Mix II is added to each sample and pipetted into the sample loading ports of a TaqMan® Array Card, greatly simplifying assay set-up and minimizing variability due to pipetting.
TaqMan® Array MicroRNA Cards
|
TaqMan® Universal Master Mix II
|
Recommended Products