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Invitrogen Collibri whole-genome library prep kits improve library prep success for high-throughput Illumina™ next-generation sequencing (NGS) systems.
Variant detection is consistent among low abundance (1 ng) and high abundance (500–1,000 ng) samples to confidently analyze variable samples.
High-quality genomic coverage from nasopharyngeal, oropharyngeal, and diverse samples increases accuracy in SARS-CoV-2 research such as the study of viral evolution and transmission patterns. The optimized workflow for SARS-CoV-2 reduces time and sequencing costs while providing flexibility to use available reagents.
Sequencing studies can now accept a greater diversity of samples and retain confidence in genomic interpretation. Variant detection rates by the Invitrogen Collibri PS DNA Library Prep Kits for Illumina Systems are similar between amplified
Figure 1. Coriell NA12878 DNA was converted into a library and sequenced on an Illumina™ NovaSeq™ 6000 system at 372M reads per sample. SNP recall calculated using FreeBayes v1.2.0.
Consistency of variant detection provides more consistent, predictable results from diverse samples. Variant detection rates by the Invitrogen Collibri ES DNA Library Prep Kits for Illumina Systems are similar between amplified (100 ng) libraries and PCR-free (100 ng) libraries.
Figure 2. Coriell NA12878 DNA was converted into a library and sequenced on an Illumina NovaSeq 6000 system at 258M reads per sample. SNP recall calculated using FreeBayes v1.2.0.
“…in a 1/10th reaction with Collibri preps, you can see really tight performance.”
Dr. Stuart Levine, Director of the BioMicro Center, MIT
![]() Collibri PS DNA Library Prep Kits for Illumina Systems | ![]() Collibri ES DNA Library Prep Kits for Illumina Systems | |
PCR-free input | 500 ng | 100 ng |
Input | 1–1,000 ng | 1–500 ng |
Lowest GC bias | ||
DNA shearing method | Physical shearing (e.g., Covaris) | Enzymatic shearing |
Species supported | All | All |
PCR-free assay time (hr) | 1.5 | 1.5 |
Assay time (hr) | 2.75 | 2.5 |
PCR-free hands-on time (hr) | 0.5 | 0.5 |
Hands-on time (hr) | 1 | 1 |
Target insert size (bp) | 350-550 | 350-550 |
All components included | ||
Visual feedback | ||
View performance data | View performance data |
Choose between DNA fragmentation options based upon the need for even genomic coverage, high yields, and ease-of-automation.
For Research Use Only. Not for use in diagnostic procedures.