Gibco Mouse Embryonic Fibroblasts

Gibco Mouse Embryonic Fibroblasts are:

• Rigorously tested (Table 1) for consistently high quality (Figure 1 and 2, 3)
• Pre-inactivated and ready-to-use to help save time (Figure 4)
• Available in different strains, sizes and treatments to fit your needs (Tables 2 and 3)

Table 1. Tests performed during quality assessments of Gibco MEFs.

High quality

Gibco MEFs support healthy PSC cultures that form compact colonies with defined edges and express pluripotency markers (Figures 1 and 2). In addition, Gibco MEFs help ensure superior results in many applications, including reprogramming (Figure 3).

Figure 3. High-quality Gibco MEFs yield superior results in applications such as reprogramming.  Human fibroblasts were reprogrammed with the CytoTune-iPSC 2.0 Sendai Reprogramming Kit and plated on Gibco CF1 MEFs or low quality CF1 MEFs. Gibco MEFs enabled double the reprogramming efficiency compared to low quality MEFs. Images show whole wells stained with alkaline phosphatase (red).

Pre-inactivated and ready-to-use

Gibco MEFs allow you to save more than 2 weeks (compared to the typical MEF preparation time, Figure 4), and our careful preparation and quality tests help ensure you have more confidence in your feeder-dependent cultures. 

Several varieties of Gibco MEFs are available to support specific applications and different individual preferences.

Table 2. Different Gibco MEFs and recommended uses.

Locate your MTI-GlobalStem MEF product in the table below to find the equivalent or most closely related Gibco MEF products as well as some selected citations.

Table 3. MTI-GlobalStem MEFs.

* These MTI-GlobalStem MEF products do not have direct equivalents at this time, but we suggest the listed related products.

Choose Gibco media systems for superior feeder-dependent ESC/iPSC culture