7900HT Fast Real Time PCR System Support - Troubleshooting

The 7900HT Fast Real-Time PCR System software requires that the endogenous control be run on every plate within a study. If not, you will only see RQ values for the plate that contains the endogenous control on it. The other plates will have Ct values for all the wells, but the software will not calculate RQ values. If you designed your experiments in this type of layout, you will want to do your analysis in the free DataAssist™ Software instead. This program does not have the endogenous control requirement for every plate. Simply export the study results file from the 7900HT software as a *.txt or *.csv file, and use that to create a new study in the DataAssist™ software. You should now be able to see RQ values for all samples, even those without an endogenous control on the same plate.

SDS v2.4 and above allow you to convert your AQ files to RQ and vice versa.

To do so, simply go to: Tools → AQ RQ Converter

• Choose from the dropdown which direction you want to go (AQ to RQ, or RQ to AQ).
• Next click on ‘Add’ and browse to the plate file location in the new window. and click ‘Open’.
• Click on ‘Convert’. A new file will be created in the same location as the original, but with _AQ or _RQ at the end of the filename.

If you do not have v2.4, you can upgrade your software here.

One benefit of this conversion feature is that it allows you to convert 7900 files to the correct type to do analysis for relative quantification study—for example, if a plate is originally run as AQ instead of RQ, or if you would like to generate a standard curve quantification in the AQ if a plate was originally run as RQ.