Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Your gene of interest may not be expressed at a high level in your sample type, or the sample types you extract from yield very low nucleic acid, e.g., obtained by cell sorting, LCM, or FFPE.
For guidance on how to assess the amount of RNA input for your real-time PCR study, refer to page 25 of the Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR.
Solutions:
Publication: Noutsias, M. et al. (2008) Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies. BMC Mol Biol 9(1):3
For Research Use Only. Not for use in diagnostic procedures.