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Technical Reference Library
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The Molecular Probes Handbook
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Technical Notes and Product Highlights
Technical Notes and Product Highlights
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Technical Notes and Product Highlights
Definitions of Data Table Contents
The Alexa Fluor Dye Series—Note 1.1
Fluorescence Resonance Energy Transfer FRET—Note 1.2
Fluorescence Correlation Spectroscopy (FCS)—Note 1.3
Fluorescence Polarization (FP)—Note 1.4
Fluorescent Probes for Two-Photon Microscopy—Note 1.5
Antibody Structure and Classification—Note 7.1
MHC Tetramer Technology—Note 7.2
SYBR Safe DNA Gel Stain—Note 8.1
Monitoring Protein-Folding Processes with Environment-Sensitive Dyes—Note 9.1
Labeling Small Peptides with Amine-Reactive Dyes in Organic Solvents—Note 9.2
BacMam Gene Delivery and Expression Technology—Note 11.1
Using Organic Fluorescent Probes in Combination with GFP—Note 12.1
Mitochondria in Diseases—Note 12.2
Lipid-Mixing Assays of Membrane Fusion—Note 13.1
Antibodies for Detecting Membrane-Surface Labels—Note 13.2
Anti–Lucifer Yellow Dye, Anti–Alexa Fluor 405/Cascade Blue Dye and Anti–Alexa Fluor 488 Dye Antibodies—Note 14.1
Fluorescent Probes for Photoconversion of Diaminobenzidine Reagents—Note 14.2
Assays of Volume Change, Membrane Fusion and Membrane Permeability—Note 14.3
G Proteins and GTP Analogs for Binding Studies—Note 17.1
Loading and Calibration of Intracellular Ion Indicators—Note 19.1
MAXC Computer Program for Calculating Free Ca2+ Concentrations—Note 19.2
Using the Fluorescence SpectraViewer—Note 23.1
Selecting Optical Filters for Fluorescence Microscopy—Note 23.2
Molecular Probes Handbook
Table of Contents
Fluorescence Fundamentals
Chapter 1—Fluorophores and Their Amine-Reactive Derivatives
Chapter 2—Thiol-Reactive Probes
Chapter 3—Click Chemistry and other Functional Group Modifications
Chapter 4—Biotin and Hapten Derivatives
Chapter 5—Crosslinking and Photoactivatable Reagents
Chapter 6—Ultrasensitive Detection Technology
Chapter 7—Antibodies, Avidins and Lectins
Chapter 8—Nucleic Acid Detection and Analysis
Chapter 9—Protein Detection and Analysis
Chapter 10—Enzyme Substrates and Assays
Chapter 11—Probes for Cytoskeletal Proteins
Chapter 12—Probes for Organelles
Chapter 13—Probes for Lipids and Membranes
Chapter 14—Fluorescent Tracers of Cell Morphology and Fluid Flow
Chapter 15—Assays for Cell Viability, Proliferation and Function
Chapter 16—Probes for Endocytosis, Receptors and Ion Channels
Chapter 17—Probes for Signal Transduction
Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide
Chapter 19—Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions
Chapter 20—pH Indicators
Chapter 21—Indicators for Na+, K+, Cl– and Miscellaneous Ions
Chapter 22—Probes for Membrane Potential
Chapter 23—Antifades and Other Tools for Fluorescence Applications
List of Tables
Technical Notes and Product Highlights
Chapter 1
The Alexa Fluor Dye Series—Note 1.1
Fluorescence Resonance Energy Transfer (FRET)—Note 1.2
Fluorescence Correlation Spectroscopy (FCS)—Note 1.3
Fluorescence Polarization (FP)—Note 1.4
Fluorescent Probes for Two-Photon Microscopy—Note 1.5
Chapter 7
Antibody Structure and Classification—Note 7.1
MHC Tetramer Technology—Note 7.2
Chapter 8
SYBR Safe DNA Gel Stain—Note 8.1
Chapter 9
Monitoring Protein-Folding Processes with Environment-Sensitive Dyes—Note 9.1
Labeling Small Peptides with Amine-Reactive Dyes in Organic Solvents—Note 9.2
Chapter 11
BacMam Gene Delivery and Expression Technology—Note 11.1
Chapter 12
Using Organic Fluorescent Probes in Combination with GFP—Note 12.1
Mitochondria in Diseases—Note 12.2
Chapter 13
Lipid-Mixing Assays of Membrane Fusion—Note 13.1
Antibodies for Detecting Membrane-Surface Labels—Note 13.2
Chapter 14
Anti–Lucifer Yellow Dye, Anti–Alexa Fluor 405/Cascade Blue Dye and Anti–Alexa Fluor 488 Dye Antibodies—Note 14.1
Fluorescent Probes for Photoconversion of Diaminobenzidine Reagents—Note 14.2
Assays of Volume Change, Membrane Fusion and Membrane Permeability—Note 14.3
Chapter 17
G proteins and GTP Analogs for Binding Studies—Note 17.1
Chapter 19
Loading and Calibration of Intracellular Ion Indicators—Note 19.1
MAXC Computer Program for Calculating Free Ca2+ Concentrations—Note 19.2
Chapter 23
Using the Fluorescence SpectraViewer—Note 23.1
Selecting Optical Filters for Fluorescence Microscopy—Note 23.2
For Research Use Only. Not for use in diagnostic procedures.