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Vanquish Online SPE HPLC and UHPLC Systems
Ensure confidence in complex sample analysis using the Thermo Scientific Vanquish Online SPE HPLC and UHPLC Systems. These systems perform analyte pre-concentration and sample clean-up using an automated, repeatable workflow while saving solvent and time compared to offline sample preparation steps. The system is configured using modules from the Vanquish Core HPLC, Vanquish Flex UHPLC or Horizon UHPLC systems, retaining all the performance, robustness and ease of use of the Vanquish LC platform.
Highlights
Greater confidence
Automated online solid-phase extraction (SPE) provides maximum repeatability and confidence for challenging samples by trapping and enriching the analyte to remove unwanted sample matrix, such as salts and other contaminants, before loading onto the analytical column.
Flexibility
The modularity of the Vanquish LC systems makes it easy to build a system to best meet your application requirements with flexibility to later upgrade a standard HPLC instrument to a more advanced configuration for more complex analyses.
Save time and resources
The automated workflow, with predefined trap and elution steps in the instrument method, allows for unattended sample analysis while reducing human error, saving time and solvent compared to offline approaches.
Easy and reliable tool-free connections
Vanquish Online SPE HPLC and UHPLC systems are fitted with Thermo Scientific Viper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.
CDS software
The Vanquish HPLC and UHPLC systems seamlessly integrate with our Thermo Scientific Chromeleon Chromatography Data System (CDS) to deliver superior compliance tools, networking capabilities, automation and data processing.
What is online SPE for HPLC and UHPLC?
The online SPE system allows you to automate sample clean up to remove the sample matrix via a bidirectional trap column prior to being eluted for separation on the analytical column.
The flow scheme illustrates how the six-port valve is used to switch the trap column for sample loading and elution.
Videos
System specifications
The table below provides an overview of the Vanquish Online SPE HPLC and UHPLC system specifications for standard setups. Not all specifications are listed. Specifications may differ based on the specific system components selected. See detailed Component Specifications for more information.
| Category | Vanquish Online SPE HPLC and UHPLC Systems |
| System pressure, maximum | 700, 1000 or 1500 bar, depends on system configuration |
| Flow range | 0–5, 0-8 or 0-10 mL/min, depends on pumps selected |
| Pump options (default gradient delay volume) | Binary (35, 200, or 400 µL) Dual (679 µL) Delay volume depends on pump(s) selected |
| Biocompatible | Yes, depends on system configuration |
| pH range | 2–12, chloride concentration up to 1 mol/L |
| System design | Modular |
| Touchscreen user interface display compatible (optional) | Yes, depends on system configuration |
| Solvent monitoring system compatible (optional) | Yes, depends on system configuration |
| Customizable gradient delay volume to simplify method transfer | Yes, depends on system configuration |
| SmartInject technology for maximum injection precision and improved column lifetime | Yes |
| Injection volume range | Standard: Up to 0.01-25 or 0.01-100 µL (Vanquish Flex/Vanquish Horizon) |
Sample cooling |
Yes, 4-40°C Depends on system configuration |
Sample capacity (standard) |
4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available + capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel |
| Sampler extension available (optional) | Yes, with Vanquish Charger |
| Column compartment thermostatting | Yes, 5-120°C, depends on column compartment selected |
Column compartment pre-heater |
Active and passive |
Column compartment post-column cooler |
Yes Depends on column compartment selected |
| Detector choices | Vanquish Charged Aerosol Detector Vanquish Diode Array Detector Vanquish Fluorescence Detector Vanquish Multiple Wavelength Detector Vanquish Variable Wavelength Detector Refractive Index Detector Mass Spectrometer |
Component specifications
Standard Vanquish Online SPE HPLC and UHPLC systems are made up of the following components. Download the associated specifications sheet for more detail on each component.
| Vanquish Horizon Online SPE UHPLC System | Vanquish Flex Online SPE UHPLC System | Vanquish Core Online SPE HPLC System | |
Pump
|
2 Vanquish Binary Pump H or 1 Vanquish Binary Pump H and 1 Vanquish Quaternary Pump F |
2 Vanquish Binary Pump F or 2 Vanquish Quaternary Pump F or 1 Vanquish Binary Pump F and 1 Vanquish Quaternary Pump F or 1 Vanquish Dual Pump F |
2 Vanquish Binary Pump C or 2 Vanquish Quaternary Pump C or 1 Vanquish Binary Pump C and 1 Vanquish Quaternary Pump C or 1 Vanquish Dual Pump C |
Autosampler
|
Vanquish Split Sampler HT | Vanquish Split Sampler FT | Vanquish Split Sampler C or Vanquish Split Sampler CT |
Extended sample capacity (optional)
|
Vanquish Charger (optional) |
||
Column compartment
|
Vanquish Column Compartment H | Vanquish Column Compartment C |
|
Virtually zero dead volume system connections
|
Viper UHPLC Fittings | ||
Detectors
|
Visit our HPLC and UHPLC Detectors page | ||
