Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein L is a recombinant protein expressed in Escherichia coli (35.8 kDa; 4 IgG binding sites) and is recommended for human or mouse monoclonal antibodies known to have appropriate kappa light chains.

We offer two base supports and a variety of formats to purify antibodies using the Protein L ligand. Our portfolio is designed to meet small-scale (screening) to pilot-scale needs.

  • Multiple formats—magnetic beads, loose resin, spin columns and kits, and FPLC cartridges enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein L product for antibody purification

Product links in the last row of each column

 Pierce Protein L
Magnetic Beads		Pierce Protein L
Agarose		Pierce Protein L Plus
Agarose
Bead or resin size1 μm45–165 μm45–165 μm
Binding capacity≥110 μg human IgG/mg beads5–10 mg human IgG/mL resin10–20 mg human IgG/mL resin
Maximum linear flow rateN/A700 cm/hr700 cm/hr
SupportMagnetite coated polymerCrosslinked 6% beaded agaroseCrosslinked 6% beaded agarose
Recommended application scalescreeningbatch, pilotbatch, pilot
Formats availableloose beadsloose beads, pre-packed spin columns and kits, chromatography cartridgesloose beads
Order product(s)8884920510
(loose resin)
89928
(1 mL cartridge)
89929
(5 mL cartridge)		20520
(loose resin)
Resources

For Research Use Only. Not for use in diagnostic procedures.