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Fundamentals of Fluorescence Microscopy
Creating snapshots of cell structure and function using light
Learn the basic physics behind the excitation and emission of a fluorophore and how microscopes channel and capture the light generated to make a fluorescence-based image.
Physical properties that define fluorescence
Learn about basic properties of light, fluorophore excitation, emission, and Stokes shift.
How fluorescence microscopy works
Understand advantages of fluorescence microscopy compared to brightfield, the differences between magnification and resolution, and how using fluorescence can help increase resolution.
Epifluorescence microscope basics
Learn about the light path in typical epifluorescence microscopes and their limits of resolution
Using filters to capture your signal
Understand why filters are used and where in the light path you will find them.
Understanding your objective
Understand what the code on your objective means, why numerical aperture matters, and ways to make sure your microscope configuration will work for your experiment
Now you can take the Molecular Probes School of Fluorescence Basics of Imaging: Chapter 1—Fundamentals of fluorescence microscopy with you on your devices.
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For Research Use Only. Not for use in diagnostic procedures.