NovaFluor Red 685 excitation shown as dotted line and emission shown as solid red histogram
4640695/40637685(in buffer) 5
flow cytometry

Invitrogen NovaFluor Red 685 dye is ~4 times brighter than APC-Cyanine 5.5 and Alexa Fluor 680 dye. In complex multicolor flow cytometry panels, NovaFluor Red 685 dye can be used to fill the spectral gap between Alexa Fluor 647 and Alexa Fluor 700 dyes.

The macromolecule-based NovaFluor Red 685 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Red 685 primary antibodies

Benefits of NovaFluor Red 685 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Bright fluorescence allows it to be paired with medium-to-low abundance antigens
  • Can be used to fill the spectra gap between Alexa Fluor 647 and Alexa Fluor 700 dyes
  • Can be used in a flow cytometry panel with NovaFluor Red 660 (or APC-Alexa Fluor 647) and Alexa Fluor 700 dyes
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Red 685 dye

Spectral signature of NovaFluor Red 685 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 685 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.

Side scatter plot with unstained and CD16 NovaFluor Red 685 dye-stained normal human PBMCs
Staining of human peripheral blood cells with NovaFluor Red 685 conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD16 Monoclonal Antibody, NovaFluor Red 685 (right). Data was acquired in the R3 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.

Additional resources

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