Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Search Thermo Fisher Scientific
Plasmid DNA Isolation |
我們的Invitrogen質體分離試劑盒提供您更多選擇,以降低多類型樣品處理過程所帶來的挑戰。我們的客製質體DNA萃取產品,可以依照您需要的純度和通量來分離質體DNA。我們提供一系列高效能且經濟實惠的質體純化技術,協助您克服常見的質體製備難題,如低回收率和過多雜質的問題。觀看我們的質體分離教學影片,了解如何使用Invitrogen PureLink和Thermo Scientific GeneJET萃取試劑盒來純化質體DNA。
Plasmid purification, also known as plasmid isolation or plasmid DNA isolation, refers to the process of extracting and purifying plasmid DNA from bacterial cells, allowing scientists to obtain a highly concentrated and pure form of the plasmid for further analysis and experimentation. The key steps in plasmid purification typically include bacterial cell lysis, removal of cellular debris, binding of plasmid DNA, washing, elution of plasmid DNA, and optional additional steps depending on the downstream application.
分子生物等級的快速分離 | 達黃金標準的超高純度與品質 | 提升純度以符合高靈敏應用 | ||
---|---|---|---|---|
純度級別 | 分子 | 轉染 | 高效能轉染 | |
內毒素 | 標準 (>10 EU/µg) | 低內毒素 (0.1–1 EU/µg) | 無內毒素 (< 0.1 EU/µg) | |
質體DNA純化試劑盒 | ![]() GeneJET | ![]() PureLink HiPure | ![]() PureLink Fast | ![]() PureLinkExpi Endotoxin-Free |
總反應時間 | 15-60分鐘 | 30-120分鐘 | 30分鐘 | 90-120分鐘 |
製備規格 | Mini–Maxi | Mini–Giga | Midi, Maxi | Maxi, Mega, Giga |
Yield up to | 20 µg–1 mg | 20 µg–15 mg | 0.4 mg (midi prep) or 1.5 mg (maxi prep) | 1.5–15 mg |
技術 | 矽膠膜 | 陰離子交換 | 高效矽膠 | 陰離子交換膜 |
應用範圍 | 不靈敏
| 靈敏
| 高靈敏
| |
立即訂購 | 立即訂購 | 立即訂購 | 立即訂購 |
When it comes to plasmid isolation, scientists have a range of options depending on their specific needs. Molecular-grade plasmid isolation technologies offer speed and cost-effectiveness, making them excellent for robust applications like cloning, nucleic acid labeling, PCR, and sequencing, where high purity levels are not essential. On the other hand, transfection-grade plasmid purification technologies are designed for applications that require greater purity and higher yields, such as transfection of standard cell lines and in vitro transcription. Transfection-grade plasmid DNA can also be used for molecular-grade applications. For the most sensitive applications, such as transfection of sensitive cell lines or in vivo research, advanced transfection-grade plasmid isolation technology is recommended. This technology helps ensure the highest purity and can also be used for all molecular and transfection-grade applications.
Purity grade | Molecular | Transfection | Advanced transfection |
---|---|---|---|
Endotoxin level | >10 EU/µg | 0.1–1 EU/µg | <0.1 EU/µg |
Learn more: Plasmid DNA purity grades
為了達成下游應用需要的純度以及產量,可獲得穩定的質體DNA純化結束是蛋白質表現工作流程的關鍵步驟。了解如何獲取轉染等級的質體,接續可應用於Gibco ExpiCHO和Expi293的哺乳動物表現系統。
Explore: ExpiCHO Expression System
Automated plasmid purification refers to the use of advanced robotics, liquid handling capabilities, and specialized magnetic-bead purification kits to help streamline the isolation of plasmid DNA from bacterial cultures. These solutions can handle multiple samples simultaneously, helping increase throughput and reducing manual processing time. They also help minimize human error to enable reproducibility by precisely controlling the purification steps and volumes of reagents used. Thermo Fisher offers automated plasmid purification solutions that employ innovative technologies that allow for high-throughput, centrifuge-free purification of transfection-grade plasmid DNA with minimal hands-on steps. This purified pDNA can then be used for various downstream applications, such as transfection into sensitive cell lines, preparation of short hairpin vectors, enzymatic modifications, molecular cloning, restriction digestion, sequencing, and in vitro transcription/translation.
Applied Biosystems MagMAX Pro HT NoSpin Miniprep Plasmid Purification Kit | Thermo Scientific KingFisher Plasmid Pro | |
---|---|---|
![]() | ![]() | |
Prep sizes available | Mini | Maxi |
Purity grade/Endotoxin level | Transfection grade (0.1–1 EU/µg) | Transfection grade (0.1–1 EU/µg) |
Transfection-grade plasmid isolation kits are specifically designed for scientists who need to extract plasmid DNA (pDNA) with minimal levels of endotoxins from bacterial cultures. During the plasmid purification process, large amounts of endotoxins are released from lysed bacteria, which can negatively impact cell growth, cell viability, transfection efficiency, protein expression, and even mimic sepsis. To address these concerns, our kits designed for low-endotoxin plasmid DNA isolation carefully remove these endotoxins, helping ensure the integrity of the isolated plasmid DNA. These kits offer a fast and easy method of plasmid purification, helping provide high purity and yield for sensitive downstream applications. Whether you are working with small or large prep sizes, our transfection-grade plasmid isolation kits are tailored to help meet your specific needs, enabling you achieve reliable and reproducible results.
Invitrogen PureLink Fast Low-Endotoxin Purification Kits | Invitrogen PureLink HiPure Plasmid Kits | PureLink HiPure Plasmid Filter Kits | |
---|---|---|---|
![]() | ![]() | ![]() | |
Prep sizes available | Midi, Maxi | Mini, Midi, Maxi, Mega, Giga | Midi, Maxi |
Purity grade/Endotoxin level | Transfection grade (0.1–1 EU/µg) | Transfection grade (0.1–1 EU/µg) | Transfection grade (0.1–1 EU/µg) |
For scientists who require an even higher level of endotoxin removal, we offer advanced transfection-grade plasmid isolation kits. These kits go beyond the standard transfection-grade kits and provide an enhanced level of endotoxin removal, helping ensure the utmost purity of the isolated plasmid DNA. With our advanced transfection-grade plasmid isolation kits, you can confidently perform downstream applications that demand the highest quality pDNA. These kits are excellent for researchers working on critical experiments where even trace amounts of endotoxins can have a significant impact on their results. Using our advanced-transfection grade plasmid isolation kits can help eliminate the risks associated with endotoxins and obtain pure plasmid DNA for your scientific endeavors.
For fast, easy, and reliable plasmid purification, our Invitrogen PureLink and GeneJET molecular-grade plasmid DNA (pDNA) isolation kits produce pure, ready-to-use plasmids that are compatible with a range of standard molecular biology applications. Molecular-grade kits are suitable for transformation of bacterial and yeast cells; however, they may not be well suited for transfection of sensitive mammalian cells due to the presence of endotoxins. For transfection applications, please see the transfection-grade plasmid purification kits above.
Invitrogen PureLink Pro Quick 96 Plasmid Purification Kit | Invitrogen PureLink Quick Plasmid Miniprep Kit | Thermo Scientific GeneJET Plasmid Kits | |
---|---|---|---|
![]() | ![]() | ![]() | |
Prep size available | Small-scale 96-well plate format | Mini | Mini, Midi, Maxi |
Purity grade/Endotoxin level | Molecular grade (>10 EU/µg) | Molecular grade (>10 EU/µg) | Molecular grade (>10 EU/µg) |
觀看影片,學習如何使用PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit純化質體DNA。本影片向您展示如何分離並純化出高品質且無內毒素的質體DNA,以符合高靈敏的應用需求(如初代細胞的轉染)。
本影片展示了GeneJET Plasmid Miniprep Kit如何在15分鐘內純化出分子等級的質體DNA。分子等級的質體DNA適合應用於基因選殖、核酸標定、PCR、DNA定序和轉化實驗。
使用Invitrogen PureLink HiPure Expi Plasmid Megaprep Kit,只需90分鐘即可分離出轉染等級的質體DNA。這些試劑盒中配載了新型專利陰離子交換樹脂,具備高流速、高解析度及高效率的特性,同時獲得高產量。
了解如何使用Invitrogen PureLink HiPure Plasmid Filter Maxiprep Kit純化出可應用於轉染的質體DNA。轉染等級的質體DNA,符合所有分子層級的應用需求,如體外轉錄和轉染。
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
For Research Use Only. Not for use in diagnostic procedures.