TaqMan Drug Metabolism Genotyping Assays offer the broadest menu of drug metabolism enzyme (DME) targets on the market, offering 2,700 unique assays that detect polymorphisms in 221 genes for high-value DMEs and associated transporter targets. The products use 5´ nuclease assay chemistry to amplify and detect specific SNP alleles, multinucleotide polymorphisms (MNPs), and insertion/deletions (indels).

  • Help accurately detect biologically important polymorphisms with roles in drug metabolism and transport
  • Available as individual ready-to-use tubes or with custom plating
  • 95% coverage of ADME core markers defined by the PharmaADME consortium
  • Work confidently—our bioinformatics mapping, design, and in silico QC means you are studying the right SNP
  • Get started immediately with ready-to-use assays optimized for all Applied Biosystems real-time PCR systems

Explore DME solutions for pharmacogenomics

Experimental workflow: Find the products you need for every step

Isolate and purify genomic DNA.

Products for step 1: Prepare sample

teaser-portrait.par.75601.image.-1.0.1

BloodPrep DNA Chemistry for Cultured Cells and Blood
BloodPrep chemistry is designed to rapidly isolate DNA from fresh or frozen blood of any animal species, tissue-cultured cells, or buccal swabs. The protocol is designed for use with the Applied Biosystems 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.

Select individual DME Assays or Assay Sets.
teaser-portrait.par.66877.image.-1.0.1

TaqMan® Drug Metabolism Assays
Genotype 2,700 high-value polymorphisms located in regulatory elements and coding regions for 221 drug metabolism and transporter genes. 

Prepare reaction plate and amplify DNA using sequence specific primers and TaqMan® probe on an Applied Biosystems® real-time PCR system or thermal cycler.

Reagents & kits

taqpath-proamp-master-mix-70

TaqPath ProAmp Master Mixes*
TaqPath ProAmp Master Mixes are versatile master mixes developed for high-throughput genotyping and copy number variation protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Master Mix includes ROX™ passive reference dye for lower multiplexing of up to 3 targets.

taqpath-proamp-master-mix-70

TaqPath ProAmp Multiplex Master Mix*
TaqPath ProAmp Master Mixes are versatile master mixes developed for high-throughput genotyping and copy number variation protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Multiplex Master Mix includes MUSTANG PURPLE™ passive reference dye for higher multiplexing of up to 4 targets.

* General purpose reagents: TaqPath master mixes are general purpose reagents manufactured in an ISO 13485–certified facility and labeled "For Laboratory Use."

Instruments & accessories   

proflex-96-well-pcr-system-70

ProFlex PCR System
The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features that fit how you work today. Interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently.

simpliamp-thermal-cycler-70

SimpliAmp Thermal Cycler
The Applied Biosystems SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow. Innovations like a responsive color touch screen and Applied Biosystems VeriFlex temperature control technology enable simple, accurate optimization. 

Perform an endpoint plate read using Applied Biosystems® real-time PCR systems; TaqMan® Drug Metabolism Genotyping Assay MGB probes provide a fluorescence signal for the amplification of each allele.

Instruments & accessories   

teaser-portrait.par.32593.image.-1.0.1

StepOnePlus™ Real-Time PCR System 
The StepOnePlus™ Real-Time PCR System makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96-well, 4 color system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

teaser-portrait.par.32593.image.-1.0.1

StepOne™ Real-Time PCR System 
This 48-well low-throughput real-time PCR instrument makes it easy to get publication ready high quality data on day one. This remarkably simple real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers new to real-time PCR.

teaser-portrait.par.74776.image.-1.0.1

7500 Fast Real-Time PCR System 
The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. 

teaser-portrait.par.12433.image.-1.0.1

7900HT Fast Real-Time PCR System 
The 7900HT Fast Real-Time PCR System combines 384-well plate compatibility with fully automated robotic loading—and offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

teaser-portrait.par.97485.image.-1.0.1

ViiA™ 7 Real-Time PCR System 
The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.    

The plotted fluorescence signals indicate which alleles are in each sample. Make manual allele calls or review automatic allele calls, and then convert allele calls to genotypes.

TaqMan® Genotyper Software
TaqMan® Genotyper Software is for use with TaqMan® SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96- & 384-well microtiter plates, and OpenArray® Genotyping Plates and offers a state-of-the-art genotype calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

 Download

Step 1 : Prepare Sample
Isolate and purify genomic DNA.

Products for step 1: Prepare sample

teaser-portrait.par.75601.image.-1.0.1

BloodPrep DNA Chemistry for Cultured Cells and Blood
BloodPrep chemistry is designed to rapidly isolate DNA from fresh or frozen blood of any animal species, tissue-cultured cells, or buccal swabs. The protocol is designed for use with the Applied Biosystems 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.

Step 2 : Select Assays
Select individual DME Assays or Assay Sets.
teaser-portrait.par.66877.image.-1.0.1

TaqMan® Drug Metabolism Assays
Genotype 2,700 high-value polymorphisms located in regulatory elements and coding regions for 221 drug metabolism and transporter genes. 

Step 3 : Amplify DNA
Prepare reaction plate and amplify DNA using sequence specific primers and TaqMan® probe on an Applied Biosystems® real-time PCR system or thermal cycler.

Reagents & kits

taqpath-proamp-master-mix-70

TaqPath ProAmp Master Mixes*
TaqPath ProAmp Master Mixes are versatile master mixes developed for high-throughput genotyping and copy number variation protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Master Mix includes ROX™ passive reference dye for lower multiplexing of up to 3 targets.

taqpath-proamp-master-mix-70

TaqPath ProAmp Multiplex Master Mix*
TaqPath ProAmp Master Mixes are versatile master mixes developed for high-throughput genotyping and copy number variation protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Multiplex Master Mix includes MUSTANG PURPLE™ passive reference dye for higher multiplexing of up to 4 targets.

* General purpose reagents: TaqPath master mixes are general purpose reagents manufactured in an ISO 13485–certified facility and labeled "For Laboratory Use."

Instruments & accessories   

proflex-96-well-pcr-system-70

ProFlex PCR System
The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features that fit how you work today. Interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently.

simpliamp-thermal-cycler-70

SimpliAmp Thermal Cycler
The Applied Biosystems SimpliAmp Thermal Cycler is an easy-to use, compact, and accurate thermal cycler designed to fit every lab’s essential PCR workflow. Innovations like a responsive color touch screen and Applied Biosystems VeriFlex temperature control technology enable simple, accurate optimization. 

Step 4 : Allelic Discrimination
Perform an endpoint plate read using Applied Biosystems® real-time PCR systems; TaqMan® Drug Metabolism Genotyping Assay MGB probes provide a fluorescence signal for the amplification of each allele.

Instruments & accessories   

teaser-portrait.par.32593.image.-1.0.1

StepOnePlus™ Real-Time PCR System 
The StepOnePlus™ Real-Time PCR System makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96-well, 4 color system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

teaser-portrait.par.32593.image.-1.0.1

StepOne™ Real-Time PCR System 
This 48-well low-throughput real-time PCR instrument makes it easy to get publication ready high quality data on day one. This remarkably simple real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers new to real-time PCR.

teaser-portrait.par.74776.image.-1.0.1

7500 Fast Real-Time PCR System 
The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. 

teaser-portrait.par.12433.image.-1.0.1

7900HT Fast Real-Time PCR System 
The 7900HT Fast Real-Time PCR System combines 384-well plate compatibility with fully automated robotic loading—and offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

teaser-portrait.par.97485.image.-1.0.1

ViiA™ 7 Real-Time PCR System 
The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.    

Step 5 : Analyze Data
The plotted fluorescence signals indicate which alleles are in each sample. Make manual allele calls or review automatic allele calls, and then convert allele calls to genotypes.

TaqMan® Genotyper Software
TaqMan® Genotyper Software is for use with TaqMan® SNP Genotyping Assays (Predesigned, Custom & DME) in combination with 48-, 96- & 384-well microtiter plates, and OpenArray® Genotyping Plates and offers a state-of-the-art genotype calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.

 Download

Resources

For Research Use Only. Not for use in diagnostic procedures.

* General purpose reagents: TaqPath master mixes are general purpose reagents manufactured in an ISO 13485–certified facility and labeled "For Laboratory Use."